Maternal Estrogen Receptor-β Expression during Mouse Gestation


  • C.Y. SPONG,

  • S.K. MCCUNE,



Address reprint requests to Catherine Y. Spong, M.D., Section of Developmental and Molecular Pharmacology, LDN NICHD, NIH, Building 49, 5A-38, MSC 4480, 9000 Rockville Pike, Bethesda, MD 20892.



PROBLEM: Although estrogen receptor (ER)-α has been well characterized, the recently identified novel ER-β has not. In some tissues, there is overlap of the ERs, which allows for rescue in cases of deficiency; in other tissues, the ERs appear to have opposite effects. The objective of this study was to evaluate the expression of ER-β during pregnancy.
METHOD OF STUDY: Pregnant mouse uteri (embryonic days 6–14, 16, 18) were studied. ER-α and ER-β oligonucleotide probes were end-labeled and in situ hybridization histochemistry was performed.
RESULTS: ER-β was strongly expressed in maternal ovaries; there was no other evidence of strong expression during gestation. ER-α was expressed in the uterus throughout gestation, with decreasing intensity as gestation progressed, and in maternal ovarian tissue.
CONCLUSIONS: Differential expression of the two ERs was apparent during pregnancy, with ER-α playing a dominant role. This may have implications for selective drug treatment targeting estrogen receptors.