Chemotactic Activity for Polymorphonuclear Leukocytes: Meconium Versus Meconium-Stained Amniotic Fluid


Address reprint requests to Tetsuo Yamada, M.D., Department of Obstetrics and Gynecology, Jichi Medical School, Minamikawachi-machi, Tochigi, 329-0498, Japan.



PROBLEM: Our previous study indicated that meconium-stained amniotic fluid (turbid AF) possessed a potent chemotactic activity for leukocytes, which may be dependent on interleukin-8. It is not known, however, whether meconium itself possesses this chemotactic activity.
METHOD OF STUDY: Meconium samples were collected from mature neonates with and without turbid AF. A 5% meconium suspension in phosphate buffered saline was prepared and measured for its chemotactic activity for leukocytes using the blind well chamber technique. Concentrations of IL-8, TNFα and IL-1β were also measured with ELISA.
RESULTS: The number of leukocytes that migrated to the meconium suspension (35±27) was comparable with that of the clear AF (31±37), but was significantly lower than that of the turbid AF (184±62, P<0.0001). The meconium suspension contained much lower levels of IL-8, TNFα and IL-1β than the turbid AF.
CONCLUSIONS: Meconium itself exhibits a lower chemotactic activity for polymorphonuclear leukocytes than turbid AF in vitro. The leukocyte chemotactic activity of turbid AF does not originate from meconium itself.