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Keywords:

  • Amniotic fluid;
  • chemotaxis;
  • interleukin-8;
  • leukocyte;
  • meconium

PROBLEM: Our previous study indicated that meconium-stained amniotic fluid (turbid AF) possessed a potent chemotactic activity for leukocytes, which may be dependent on interleukin-8. It is not known, however, whether meconium itself possesses this chemotactic activity. METHOD OF STUDY: Meconium samples were collected from mature neonates with and without turbid AF. A 5% meconium suspension in phosphate buffered saline was prepared and measured for its chemotactic activity for leukocytes using the blind well chamber technique. Concentrations of IL-8, TNFα and IL-1β were also measured with ELISA. RESULTS: The number of leukocytes that migrated to the meconium suspension (35±27) was comparable with that of the clear AF (31±37), but was significantly lower than that of the turbid AF (184±62, P<0.0001). The meconium suspension contained much lower levels of IL-8, TNFα and IL-1β than the turbid AF. CONCLUSIONS: Meconium itself exhibits a lower chemotactic activity for polymorphonuclear leukocytes than turbid AF in vitro. The leukocyte chemotactic activity of turbid AF does not originate from meconium itself.