A Pr3+-doped transparent oxyfluoride glass-ceramic containing Ca5(PO4)3F nanocrystals was prepared by melt quenching and subsequent thermal treatment. The crystallization phase and morphology of the Ca5(PO4)3F nanocrystals were investigated by X-ray diffraction and transmission electron microscope, respectively. The volume fraction of the Ca5(PO4)3F nanocrystals in the glass-ceramic is about 10% and the fraction of Pr3+ ions incorporated into the Ca5(PO4)3F nanocrystals is about 22%. The peak absorption cross sections at 435 and 574 nm increase up to 128% and 132% after crystallization, respectively. The peak stimulated emission cross sections of the 3P0→3H4 blue laser channel and 3P0→3F2 red laser channel for the glass-ceramic are 4.95 × 10−20 and 29.8 × 10−20 cm2, respectively. The spectral properties indicate that the glass-ceramic is a potential visible laser material.