Effects of the feeding level on juvenile Solea solea (Linnaeus, 1758) lipid-related indicators of nutritional condition were assessed in a 25-day trial experiment. Morphometric indices (K and HSI), liver lipid content and fatty acids (FA) clusters were good indicators of the feeding level, while individual FA showed highly dynamic variation patterns. Additionally, liver indices were more sensitive to feeding stress than were muscle measurements. HSI determination (and K) is fairly straightforward, with lower costs and simple procedures. Nevertheless, liver lipid content is also an important indicator of the feeding level and constitutes a direct measurement of food utilization and energy storage capacity. The individual variability and complex dynamics of FA with the feeding level are disadvantageous for use in FA analysis even for early-warning signals of feed limitation. Overall, S. solea nutritional condition can be suitably assessed by morphometric indices and lipid content, especially considering liver measurements (HSI and liver lipid content), provided that the fish development stage is accounted for (i.e. gonad development and reproductive cycle).