The effect of selected hormonal preparations, i.e. [(D-Ala6, Pro9-NEt)-mGnRH+metoclopramide] (Ovopel), [(D-Arg6, Pro9-NEt)-sGnRH+domperidone] (Ovaprim), luteinizing hormone releasing hormone (LHRHa), human chorionic gonadotropine (hCG) and carp pituitary extract (CPE) on the total volume of milt (TVM, ml), volume of milt per kg of body weight (VOM, ml kg−1 b.w.), sperm concentration (×109 ml−1) and total sperm production (TSP, ×109) of dace Leuciscus leuciscus (L.) has been examined. The control group (Control) included fish which milt was taken after 0.9% NaCl injection. The parameters of sperm movement were analysed with the CASA system and included: sperm motility (MOT, %), progressive motility (PRG, %), curvilinear velocity (VCL, μm s−1) and straightlinear velocity (VSL, μm s−1) of sperm, linearity of movements (LIN = 100 × VSL/VCL,%), wobbling (WOB, %), amplitude of lateral head displacement (ALH, μm) and beat cross frequency (BCF, Hz). Milt was taken from all fish (n = 12 per group) 48 h after hormonal stimulation and selected milt and sperm parameters were determined. The best results in volume of milt was found after Ovaprim treatment and TVM and VOM values were statistically higher compared to the values recorded in the Control (P < 0.001), LHRHa (P < 0.001), hCG (P < 0.001) and Ovopel group (P < 0.05). Ovaprim treatment significantly increased also TSP values without differences only between Ovopel group. Sperm concentration was significantly lower after Ovaprim treatment compared to the Control (P < 0.001) and Ovopel (P < 0.05). In CASA parameters, no significant differences were found between MOT, PRG, LIN, WOB and BCF values depending on the hormonal preparation used (P > 0.05). The highest VCL and VSL values were recorded after LHRHa treatment but there was no statistical differences between Control, Ovaprim and CPE groups. Significant differences in ALH parameter were recorded only between LHRHa and hCG (P < 0.01).