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Summary

The effects of three different dosages of Dimethyl-sulf-oxide (DMSO) (7, 10, 15%) were investigated on post-thaw motility of frozen spermatozoa from the shabout (Barbus grypus H., 1843). Three aliquots were diluted in each DMSO treatment at a final proportion of (10% sperm, 83% glucose extender and 7% DMSO), (10% sperm, 80% glucose extender and 10% DMSO) and (10% sperm, 75% glucose extender and 15% DMSO), respectively. Compared to fresh milt, cryopreserved spermatozoa showed a significant decrease in sperm motility. Semen frozen with the extender 1 and 3 containing 7 and 15% DMSO showed a lower percentage of motile cells (36.56 ± 1.14 and 20.89 ± 0.86%) than the extender 2 containing 10% DMSO (41.28 ± 0.83%), and this difference was significant (P < 0.05). Similarly, the extender containing 10% DMSO gave the best fertility rate (36.12 ± 1.57%).