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Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing

Authors

  • By Q. Zeng,

    1. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China
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    • Equal contributors
  • H. Ye,

    1. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China
    2. College of Life Sciences and Food Engineering, Yibin University, Yibin, China
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    • Equal contributors
  • A. Ludwig,

    1. Department of Evolutionary Genetics, Institute for Zoo and Wildlife Research, Berlin, Germany
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  • Z. Wang,

    1. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China
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  • Y. Zhang,

    1. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China
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  • Z. Peng

    Corresponding author
    1. Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China
    • Author's address: Zuogang Peng, Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Science, Chongqing 400715, China.

      E-mail: pengzuogang@gmail.com

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Summary

The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri-, tetra-, penta-, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one-eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.

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