A. Lü and X. Hu contributed equally to this work.
Proteomic analysis of differential protein expression in the skin of zebrafish [Danio rerio (Hamilton, 1822)] infected with Aeromonas hydrophila
Article first published online: 24 AUG 2013
© 2013 Blackwell Verlag GmbH
Journal of Applied Ichthyology
Volume 30, Issue 1, pages 28–34, February 2014
How to Cite
Lü, A., Hu, X., Wang, Y., Ming, Q., Zhu, A., Shen, L. and Feng, Z. (2014), Proteomic analysis of differential protein expression in the skin of zebrafish [Danio rerio (Hamilton, 1822)] infected with Aeromonas hydrophila. Journal of Applied Ichthyology, 30: 28–34. doi: 10.1111/jai.12318
- Issue published online: 8 JAN 2014
- Article first published online: 24 AUG 2013
- Manuscript Accepted: 5 JUN 2013
- Manuscript Received: 14 MAY 2013
- National Natural Science Foundation of China. Grant Numbers: 31272692, 30800847, Jiangsu Government Scholarship for Overseas Studies
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Skin is an important component of the mucosal immune system in fish, however its molecular mechanism at the proteome-level remains unclear. In this study, differentially expressed proteins in the skin of zebrafish Danio rerio responding to Aeromonas hydrophila infection were identified using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) analysis. The results showed that 17 proteins were differentially expressed, six proteins of which were up-regulated and 11 proteins down-regulated. MHC class II DR α 1 domain, immunoglobulin heavy chain V-region, glyceraldehyde-3-phosphate dehydrogenase, transcription factor AP-2-α, and actin were considered immune-related molecules in the skin of zebrafish. Expression changes of 17 proteins were further determined by zebrafish cDNA microarray analysis. This is the first known report on proteome of fish skin against A. hydrophila, which will contribute to the understanding of the mucosal immune response, bacterial disease control and prevention.