Cryopreservation of giant grouper Epinephelus lanceolatus (Bloch, 1790) sperm

Authors

  • B. Fan,

    1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China
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    • These authors contributed equally to this work.
  • X.-C. Liu,

    1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China
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    • These authors contributed equally to this work.
  • Z.-N. Meng,

    Corresponding author
    1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China
    • Author's address: Zi-Ning Meng, State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.

      E-mail: mengzn@mail.sysu.edu.cn

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  • B. H. Tan,

    1. Duke-NUS Graduate Medical School, Outram Park, Singapore
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  • L. Wang,

    1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China
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  • H.-F. Zhang,

    1. Daya Bay Fisheries Development Centre, Huizhou, China
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  • Y. Zhang,

    1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China
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  • Y.-X. Wang,

    1. Daya Bay Fisheries Development Centre, Huizhou, China
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  • H.-R. Lin

    1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China
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Summary

The grouper, Epinephelus lanceolatus, is a vulnerable species of high economic value. An effective protocol was developed for the cryopreservation of E. lanceolatus by comparing different extenders produced by mixing various cryoprotectants (dimethyl sulfoxide, methanol and glycerol) and diluents (MPRS, TS-2, TS-19, Cortland and Hank's). Using computer-assisted sperm analysis (CASA) and morphological analysis, the sperm motility and fertilization rates from post-thaw sperm were comparable to untreated controls. The results revealed that MPRS (containing 12% DMSO) or TS-19 (containing 12% DMSO), were the optimum extenders for protecting the sperm from cryo-damage in liquid nitrogen. The post-thaw sperm maintained high motility (90.61 ± 3.03%) and a fertilization rate (92.27 ± 2.43%) similar (P > 0.05) to fresh sperm (94.34 ± 4% and 94.10 ± 1.87%). This study is the first to report on the successful sperm cryopreservation of E. lanceolatus and provides an important tool for repopulating this species through aquaculture.

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