Stability of human enteric viruses in seawater samples from mollusc depuration tanks coupled with ultraviolet irradiation

Authors

  • A. de Abreu Corrêa,

    Corresponding author
    • Laboratório de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, UFSC, Florianópolis, Brazil
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  • D.S.M. Souza,

    1. Laboratório de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, UFSC, Florianópolis, Brazil
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  • V. Moresco,

    1. Laboratório de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, UFSC, Florianópolis, Brazil
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  • C.R. Kleemann,

    1. Laboratório de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, UFSC, Florianópolis, Brazil
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  • L.A.T. Garcia,

    1. Laboratório de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, UFSC, Florianópolis, Brazil
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  • C.R.M. Barardi

    1. Laboratório de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, UFSC, Florianópolis, Brazil
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Correspondence

Adriana de Abreu Corrêa, Laboratório de Virologia Aplicada, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, UFSC, Florianópolis 88040-970, Santa Catarina, Brazil.E-mail: adriabreucorrea@gmail.com

Abstract

Aims

To evaluate the stability in seawater of human adenovirus (HAdV2), murine norovirus (MNV-1) and hepatitis A virus (HAV) in a shellfish depuration system with and without ultraviolet (UV) treatment.

Methods and Results

Seawater was seeded with viruses and disinfected using a 36 W lamp. Samples were collected at 24, 48, 72, 96 and 120 h; viruses were concentrated and the viral decay was evaluated using molecular and cell culture methods. Based on the molecular results, at 120 h of disinfection, there was a reduction of more than 3 log10 for HAdV2 and HAV; MNV-1, a 4·5 log10 reduction was observed at 72 h. Infectious MNV-1 was not detected after 72 h of treatment; while HAdV2 remained infectious. Seawater not treated demonstrated a progressive viral reduction for the three viruses tested.

Conclusions

The UV reduced the number of viral particles, and the results indicate there is natural and gradual decrease of viral load and viability in seawater.

Significance and Impact of the Study

UV irradiation is the method of choice for shellfish depuration in many countries; this work showed useful information about the viral stability in seawater and application of UV to water disinfection to be used in shellfish depuration tanks.

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