Hydrocarbon-degrading potential of microbial communities from Arctic plants
Article first published online: 12 OCT 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 1, pages 71–83, January 2013
How to Cite
Ferrera-Rodríguez, O., Greer, C.W., Juck, D., Consaul, L.L., Martínez-Romero, E. and Whyte, L.G. (2013), Hydrocarbon-degrading potential of microbial communities from Arctic plants. Journal of Applied Microbiology, 114: 71–83. doi: 10.1111/jam.12020
- Issue published online: 12 DEC 2012
- Article first published online: 12 OCT 2012
- Accepted manuscript online: 17 SEP 2012 09:26PM EST
- Manuscript Accepted: 7 SEP 2012
- Manuscript Revised: 28 AUG 2012
- Manuscript Received: 15 JUN 2012
- Consejo Nacional de Ciencia y Tecnología de México
- alkB ;
- ndoB ;
To explore rhizospheric microbial communities from Arctic native plant species evaluating their bacterial hydrocarbon-degrading capacities.
Methods and Results
Eriophorum scheuchzeri, Potentilla cf. rubricaulis, Oxyria digyna, Salix arctica and Puccinellia angustata plant species were collected at Eureka (Canadian high Arctic) along with their rhizospheric soil samples. Their bacterial community fingerprints (16S rRNA gene, DGGE) were distinctive encompassing members from the phyla: Bacteroidetes, Firmicutes, Actinobacteria and Proteobacteria. Isolated diesel-degrading bacteria belonged to the phyla Actinobacteria and Proteobacteria. Strains of Mycobacterium, Nocardia, Rhodococcus, Intrasporangiaceae, Leifsoni and Arthrobacter possessed alkB and Pseudomonas possessed both ndoB and xylE gene sequences. Two Rhodococcus strains mineralized [1-14C] hexadecane at 5 and −5°C. From the rhizosphere of P. angustata, larger numbers of hydrocarbon-degrading bacteria were isolated than from other plant rhizosphere samples and all three genes alkB (from Actinobacteria), ndoB and xylE (from Pseudomonas) were detected by PCR.
(i) Arctic plants have unique rhizospheric bacterial communities. (ii) P. angustata has potential for phytoremediation research at high Arctic soils. (iii) Isolated bacteria mineralized hydrocarbons at ambient low temperatures.
Significance and Impact of the Study
To the best of our knowledge, this is the first rhizospheric exploration examining the phytoremediation potential of five Arctic plants and evaluating their microbial hydrocarbon-degrading capacities.