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Growth and survival parameter estimates and relation to RpoS levels in serotype O157:H7 and non-O157 Shiga toxin-producing Escherichia coli

  1. T.D. Mand1,
  2. D. Döpfer2,3,
  3. B. Ingham2,4,
  4. C. Ané5,6,
  5. C.W. Kaspar1,2,*

Article first published online: 19 OCT 2012

DOI: 10.1111/jam.12021

Issue

Journal of Applied Microbiology

Journal of Applied Microbiology

Volume 114, Issue 1, pages 242–255, January 2013

Additional Information

How to Cite

Mand, T.D., Döpfer, D., Ingham, B., Ané, C. and Kaspar, C.W. (2013), Growth and survival parameter estimates and relation to RpoS levels in serotype O157:H7 and non-O157 Shiga toxin-producing Escherichia coli. Journal of Applied Microbiology, 114: 242–255. doi: 10.1111/jam.12021

Author Information

  1. 1

    Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA

  2. 2

    Food Research Institute, University of Wisconsin-Madison, Madison, WI, USA

  3. 3

    Medical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, USA

  4. 4

    Department of Food Science, University of Wisconsin-Madison, Madison, WI, USA

  5. 5

    Department of Statistics, University of Wisconsin-Madison, Madison, WI, USA

  6. 6

    Department of Botany, University of Wisconsin-Madison, Madison, WI, USA

* Correspondence
Charles W. Kaspar, Department of Bacteriology, 1550 Linden Drive, University of Wisconsin-Madison, Madison, WI 53706, USA. E-mail: cwkaspar@wisc.edu.

Publication History

  1. Issue published online: 12 DEC 2012
  2. Article first published online: 19 OCT 2012
  3. Accepted manuscript online: 23 SEP 2012 08:35PM EST
  4. Manuscript Accepted: 14 SEP 2012
  5. Manuscript Revised: 13 SEP 2012
  6. Manuscript Received: 26 JUN 2012

Funded by

  • USDA National Integrated Food Safety Initiative. Grant Number: 2009-5111-20143

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FilenameFormatSizeDescription
jam12021-sup-0001-FigS1.docxWord document2716KFigure S1 Detection of RpoS via Western blotting at different growth phases. Cultures were grown in LB at 30°C. The major band, with a molecular weight of 38 kDa, represents RpoS. E = exponential phase; T = transition phase; S = stationary phase.
jam12021-sup-0002-FigS2.docxWord document183KFigure S2 Survival rate (loge CFU ml−1 day−1) in LB with 12% NaCl (aw = 0·92) and LB-HCl (pH 3·0) compared with stationary-phase RpoS levels. Point size corresponds to the ratio of RpoS level in each strain to the RpoS level of the stationary-phase Escherichia coli O157:H7 standard. The point marked with ‘a’ denotes the E. coli O157:H7 control strain.

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