These authors equally contributed to this work.
Comparison of the action of different proteases on virulence properties related to the staphylococcal surface
Article first published online: 19 NOV 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 1, pages 266–277, January 2013
How to Cite
Artini, M., Papa, R., Scoarughi, G.L., Galano, E., Barbato, G., Pucci, P. and Selan, L. (2013), Comparison of the action of different proteases on virulence properties related to the staphylococcal surface. Journal of Applied Microbiology, 114: 266–277. doi: 10.1111/jam.12038
- Issue published online: 12 DEC 2012
- Article first published online: 19 NOV 2012
- Accepted manuscript online: 12 OCT 2012 02:32AM EST
- Manuscript Accepted: 4 OCT 2012
- Manuscript Revised: 19 SEP 2012
- Manuscript Received: 1 AUG 2012
- cellular invasion;
- serine protease;
- virulence inhibition
The purpose of this study was to evaluate the antimicrobial efficacy of five different proteases belonging to two different families on Staphylococcus aureus and Staphylococcus epidermidis strains.
Methods and Results
We used three serine proteases and two metalloproteases in single species biofilm formation assays and in human cell invasion processes. Following each protease incubation with bacterial cells, surface protein patterns were analysed by SDS-PAGE and zymography. Some differently expressed proteins were identified by mass spectrometry.
The effect of tested proteases on biofilm formation was not related to the protease category but was strain-dependent and was related to the biofilm formation capacity of each staphylococcal strain. Some proteases showed a nonspecific and indiscriminate effect on surface proteins, while others induced a discrete and reproducible action on protein profiles.
Significance and Impact of the Study
The inhibition of the surface-related virulence factors is a promising avenue to overcome persistent infections caused by bacterial biofilms. To this end, we show here that proteases, in particular the metalloprotease serratiopeptidase, can interfere with adhesion and invasion of eukaryotic cells and biofilm formation in staphylococci and their use could represent a viable treatment for the development of novel combination therapies.