Exogenous ergosterol protects Saccharomyces cerevisiae from d-limonene stress
Article first published online: 19 NOV 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 2, pages 482–491, February 2013
How to Cite
Liu, J., Zhu, Y., Du, G., Zhou, J. and Chen, J. (2013), Exogenous ergosterol protects Saccharomyces cerevisiae from d-limonene stress. Journal of Applied Microbiology, 114: 482–491. doi: 10.1111/jam.12046
- Issue published online: 14 JAN 2013
- Article first published online: 19 NOV 2012
- Accepted manuscript online: 22 OCT 2012 01:50AM EST
- Manuscript Accepted: 17 OCT 2012
- Manuscript Revised: 17 SEP 2012
- Manuscript Received: 13 APR 2012
- Major State Basic Research Development Program of China. Grant Number: 2012CB720806
- National Natural Science Foundation of China. Grant Number: 31000807
- Natural Science Foundation of Jiangsu Province. Grant Numbers: BK2010150, BK2011004
- Open Project Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- citrus waste;
- Saccharomyces cerevisiae ;
- stress response
Enhancement of the tolerance of Saccharomyces cerevisiae to monoterpenes has the potential to improve the de novo biosynthesis of these chemicals as well as the efficient utilization of monoterpene-containing citrus waste. The aims of the current work are to demonstrate the mechanisms by which ergosterol, an important component of cell membranes, protects S. cerevisiae from d-limonene stress and to provide some useful information for further metabolic engineering of the yeast.
Methods and Results
Saccharomyces cerevisiae cells were treated with a sublethal dose of d-limonene for 2 h, and then ergosterol was added to investigate the physiological responses of S. cerevisiae. In d-limonene-treated cells, the membrane fluidity, permeability and saturated fatty acid ratio increased, whereas the intracellular ergosterol concentration decreased sharply. Addition of ergosterol restored membrane and intracellular ergosterol to normal levels. Exogenous ergosterol triggered nearly all of the genes that encode the biosynthesis of ergosterol.
In S. cerevisiae, the cell membrane is the target of d-limonene. Intracellular ergosterol availability is correlated with the d-limonene tolerance of the cells.
Significance and Impact of the Study
The results indicate that modification of the ergosterol biosynthesis pathway could be a promising strategy for constructing a robust yeast strain with enhanced tolerance.