A nitric oxide-releasing solution as a potential treatment for fungi associated with tinea pedis
Article first published online: 27 NOV 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 2, pages 536–544, February 2013
How to Cite
Regev-Shoshani, G., Crowe, A. and Miller, C.C. (2013), A nitric oxide-releasing solution as a potential treatment for fungi associated with tinea pedis. Journal of Applied Microbiology, 114: 536–544. doi: 10.1111/jam.12047
- Issue published online: 14 JAN 2013
- Article first published online: 27 NOV 2012
- Accepted manuscript online: 22 OCT 2012 01:50AM EST
- Manuscript Accepted: 16 OCT 2012
- Manuscript Revised: 2 OCT 2012
- Manuscript Received: 19 JUL 2012
- acidified nitrites;
- nitric oxide;
- tinea pedis
To test a nitric oxide-releasing solution (NORS) as a potential antifungal footbath therapy against Trichophyton mentagrophytes and Trichophyton rubrum during the mycelial and conidial phases.
Methods and Results
NORS (sodium nitrite citric acid) produces nitric oxide verified by gas chromatography and mass spectrometry (GC-MS). Antifungal activity of this solution was tested against mycelia and conidia of T. mentagrophytes and T. rubrum, using 1–20 mmol l−1 nitrites and 10–30 min exposure times. The direct effect of the gas released from the solution on the viability of those fungi was tested. NORS demonstrated strong antifungal activity and was found to be dose and time dependent. NO and nitrogen dioxide (NO2) were the only gases detected from this reaction and are likely responsible for the antifungal effect.
This in vitro research suggests that a single 20-min exposure to NORS could potentially be used as an effective single-dose treatment against fungi that are associated with tinea pedis in both mycelia and spore phase.
Significance and Impact of the Study
This study provides the background for developing a user-friendly footbath treatment for Athlete's Foot that will kill both vegetative fungi and its spores.