Rapid detection and respirometric profiling of aerobic bacteria on panels of selective media
Article first published online: 21 NOV 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 2, pages 423–432, February 2013
How to Cite
Jasionek, G., Ogurtsov, V. and Papkovsky, D. (2013), Rapid detection and respirometric profiling of aerobic bacteria on panels of selective media. Journal of Applied Microbiology, 114: 423–432. doi: 10.1111/jam.12049
- Issue published online: 14 JAN 2013
- Article first published online: 21 NOV 2012
- Accepted manuscript online: 25 OCT 2012 10:13AM EST
- Manuscript Accepted: 15 OCT 2012
- Manuscript Revised: 9 SEP 2012
- Manuscript Received: 6 JUL 2012
- European Commission National Development Plan and Enterprise Ireland. Grant Numbers: CFTD 05/112, CFTD 07/124, PC 08/0184
- food safety;
- high throughput;
- selective identification
To evaluate high-throughput optical oxygen microrespirometry for selective detection and predictive identification of aerobic bacteria.
Methods and Results
Using GreenLight probe, standard 384-well plates and time-resolved fluorescence reader, a representative panel of 16 partially selective media and 9 aerobic bacteria (Escherichia coli, Bacillus cereus, Staphylococcus aureus, Campylobacter jejuni, Yersinia enterocolitica, Pseudomonas aeruginosa, Streptococcus pyogenes, Salmonella typhimurium and Listeria innocua) were analysed. For each medium, bacterial strain and dilution, growth profiles were recorded, from which calibrations, doubling/generation times and growth patterns in different media were determined. Analytical performance, selectivity and general usability of the method were assessed, and mixed bacterial cultures were analysed.
The microrespirometry platform facilitates simple, real-time detection and predictive identification of aerobic bacteria by looking at the patterns of their growth and respiration in several media and determining their growth and doubling times.
Significance and Impact of the Study
The new screening method can be used for routine microbiological analysis and testing of aerobic bacterial cultures as well as complex food, environmental and clinical samples.