Prevalence and antimicrobial resistance of Enterococcus species of food animal origin from Beijing and Shandong Province, China
Article first published online: 22 NOV 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 2, pages 555–563, February 2013
How to Cite
Liu, Y., Liu, K., Lai, J., Wu, C., Shen, J. and Wang, Y. (2013), Prevalence and antimicrobial resistance of Enterococcus species of food animal origin from Beijing and Shandong Province, China. Journal of Applied Microbiology, 114: 555–563. doi: 10.1111/jam.12054
- Issue published online: 14 JAN 2013
- Article first published online: 22 NOV 2012
- Accepted manuscript online: 30 OCT 2012 02:29AM EST
- Manuscript Accepted: 22 OCT 2012
- Manuscript Revised: 13 OCT 2012
- Manuscript Received: 22 AUG 2012
- National Natural Science Foundation of China. Grant Number: 31001087
- Special Fund for Agro-scientific Research in the Public Interest. Grant Number: 201203040
- Key Projects in the National Science & Technology Pillar Program. Grant Number: 2012BAK01B02
- antimicrobial resistance;
- Enterococcus sp;
- isolation rate;
To evaluate the prevalence and antimicrobial resistance of Enterococcus species from chickens and pigs in Beijing and Shandong Province, China.
Methods and Results
Swab samples were collected from four farms in Beijing and two in Shandong Province in 2009 and tested for Enterococcus. Minimum inhibitory concentrations of antimicrobial agents were determined using broth microdilution or agar screening methods. A total of 453 Enterococcus isolates were recovered, belonging to six different Enterococcus species. All isolates were sensitive to vancomycin. Resistance to tetracycline (92·5%), amikacin (89·4%), erythromycin (72·8%) and rifampin (58·1%), and high-level streptomycin resistance (HLSR, 50·3%) were prevalent, while resistance to penicillins (7·9% to penicillin and 4·2% to ampicillin) was rare. The resistance rates to phenicols (chloramphenicol and florfenicol) and enrofloxacin, and high-level gentamicin resistance (HLGR) were approximately 30%. The vast majority of the Enterococcus isolates were classified as multidrug-resistant organisms.
Resistance of Enterococcus sp. to most antimicrobials was more prevalent in China than in European or other Asian countries.
Significance and Impact of the study
Our findings reveal a high level of antimicrobial resistance in Enterococcus isolates from food animals in China and underline the need for prudent use of antibiotics in chicken and pig production to minimize the spread of antibiotic-resistant enterococci.