Prevalence and antimicrobial resistance of Enterococcus species of food animal origin from Beijing and Shandong Province, China

Authors

  • Y. Liu,

    1. Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China
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  • K. Liu,

    1. Department of Pharmaceuticals, China Institute of Veterinary Drugs Control, Beijing, China
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  • J. Lai,

    1. Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China
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  • C. Wu,

    1. Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China
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  • J. Shen,

    1. Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China
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  • Y. Wang

    Corresponding author
    • Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China
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Correspondence

Yang Wang, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.

E-mail: wangyang@cau.edu.cn

Abstract

Aims

To evaluate the prevalence and antimicrobial resistance of Enterococcus species from chickens and pigs in Beijing and Shandong Province, China.

Methods and Results

Swab samples were collected from four farms in Beijing and two in Shandong Province in 2009 and tested for Enterococcus. Minimum inhibitory concentrations of antimicrobial agents were determined using broth microdilution or agar screening methods. A total of 453 Enterococcus isolates were recovered, belonging to six different Enterococcus species. All isolates were sensitive to vancomycin. Resistance to tetracycline (92·5%), amikacin (89·4%), erythromycin (72·8%) and rifampin (58·1%), and high-level streptomycin resistance (HLSR, 50·3%) were prevalent, while resistance to penicillins (7·9% to penicillin and 4·2% to ampicillin) was rare. The resistance rates to phenicols (chloramphenicol and florfenicol) and enrofloxacin, and high-level gentamicin resistance (HLGR) were approximately 30%. The vast majority of the Enterococcus isolates were classified as multidrug-resistant organisms.

Conclusions

Resistance of Enterococcus sp. to most antimicrobials was more prevalent in China than in European or other Asian countries.

Significance and Impact of the study

Our findings reveal a high level of antimicrobial resistance in Enterococcus isolates from food animals in China and underline the need for prudent use of antibiotics in chicken and pig production to minimize the spread of antibiotic-resistant enterococci.

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