A serological method for detection of Nosema ceranae
Article first published online: 18 DEC 2012
© 2013 The Society for Applied Microbiology No claim to US Government works
Journal of Applied Microbiology
Volume 114, Issue 3, pages 621–625, March 2013
How to Cite
Aronstein, K.A., Webster, T.C. and Saldivar, E. (2013), A serological method for detection of Nosema ceranae. Journal of Applied Microbiology, 114: 621–625. doi: 10.1111/jam.12066
- Issue published online: 18 FEB 2013
- Article first published online: 18 DEC 2012
- Accepted manuscript online: 20 NOV 2012 03:27AM EST
- Manuscript Accepted: 10 NOV 2012
- Manuscript Revised: 9 OCT 2012
- Manuscript Received: 27 JUN 2012
- USDA National Institute of Food and Agriculture
- Apis mellifera ;
- enzyme-linked immunosorbent assay;
- molecular tool;
- Nosema ceranae
We developed a new method for detection of the intracellular parasite, Nosema ceranae, one of the most economically devastating pathogens of the honeybee.
Methods and Results
The SWP-32 antibody was used for the development of an enzyme-linked immunosorbent assay (ELISA). We also compared the efficiency of this ELISA to microscopy and quantitative real-time (qRT) PCR, the methods currently in use.
ELISA is comparable in sensitivity with the qRT-PCR, less expensive and faster. When this method is commercialized and made available to bee-keepers, it will allow them to make informed decisions for the application of in-hive chemicals. Hence, bee-keepers may be able to determine when treatments for control of N. ceranae are unnecessary and reduce the cost, time and possible side effects of these treatments.
Significance and Impact of the Study
This assay provides the first serological method for detection of N. ceranae in bee colonies, which is as sensitive as DNA amplification. It can be easily adopted for both laboratory and field applications.