Detection of multiple waterborne pathogens using microsequencing arrays
Article first published online: 27 DEC 2012
© 2012 No claim to US Government works
Journal of Applied Microbiology
Volume 114, Issue 2, pages 564–573, February 2013
How to Cite
Brinkman, N.E., Francisco, R., Nichols, T.L., Robinson, D., Schaefer, F.W., Schaudies, R.P. and Villegas, E.N. (2013), Detection of multiple waterborne pathogens using microsequencing arrays. Journal of Applied Microbiology, 114: 564–573. doi: 10.1111/jam.12073
- Issue published online: 14 JAN 2013
- Article first published online: 27 DEC 2012
- Accepted manuscript online: 21 NOV 2012 02:45AM EST
- Manuscript Accepted: 31 OCT 2012
- Manuscript Revised: 10 OCT 2012
- Manuscript Received: 16 AUG 2012
- drinking water;
- environmental/recreational water;
- microbial contamination;
- water quality
A microarray was developed to simultaneously detect Cryptosporidium parvum, Cryptosporidium hominis, Enterococcus faecium, Bacillus anthracis and Francisella tularensis in water.
Methods and Results
A DNA microarray was designed to contain probes that specifically detected C. parvum, C. hominis, Ent. faecium, B. anthracis and F. tularensis. The microarray was then evaluated with samples containing target and nontarget DNA from near-neighbour micro-organisms, and tap water spiked with multiple organisms. Results demonstrated that the microarray consistently detected Ent. faecium, B. anthracis, F. tularensis and C. parvum when present in samples. Cryptosporidium hominis was only consistently detected through the use of shared probes between C. hominis and C. parvum.
This study successfully developed and tested a microarray-based assay that can specifically detect faecal indicator bacteria and human pathogens in tap water.
Significance and Impact of the Study
The use of indicator organisms has become a practical solution for monitoring for water quality. However, they do not always correlate well with the presence of many microbial pathogens, thus necessitating direct monitoring for most pathogens. This microarray can be used to simultaneously detect multiple organisms in a single sample. More importantly, it can provide occurrence information that may be used in assessing potential exposure risks to waterborne pathogens.