Comparative study of the protective capacity against Salmonella infection between probiotic and nonprobiotic lactobacilli
Article first published online: 21 DEC 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 3, pages 861–876, March 2013
How to Cite
Castillo, N.A., de Moreno de LeBlanc, A., M. Galdeano, C. and Perdigón, G. (2013), Comparative study of the protective capacity against Salmonella infection between probiotic and nonprobiotic lactobacilli. Journal of Applied Microbiology, 114: 861–876. doi: 10.1111/jam.12074
- Issue published online: 18 FEB 2013
- Article first published online: 21 DEC 2012
- Accepted manuscript online: 19 NOV 2012 12:29PM EST
- Manuscript Accepted: 10 NOV 2012
- Manuscript Revised: 24 OCT 2012
- Manuscript Received: 6 SEP 2012
- Consejo de Investigación de la Universidad Nacional de Tucumán. Grant Number: 26/D442
- Consejo Nacional de Investigaciones Científicas y Técnicas. Grant Number: PIP 0652
- lactic acid bacteria;
To investigate the immunoprotective ability of three Lactobacilli strains against Salmonella enterica serovar Typhimurium in a mouse model. To identify the probiotic properties involved in the protection against infection caused by this pathogen.
Methods and Results
The immunomodulatory effect of three different lactobacilli strains: Lactobacillus (Lact.) casei CRL 431 (probiotic bacterium), Lact. delbrueckii subsp. bulgaricus CRL 423 (Lact. bulgaricus) and Lact.acidophilus CRL 730 was compared using a mouse model of Salmonella infection. Lactobacillus casei continuous administration improved animal survival, diminished pathogen spreading outside the intestine, attenuated the intestinal inflammation, modulated cytokine profile previous and postinfection and increased the expression and secretion of IgA in the gut. Additionally, the administration of this lactobacilli increased peritoneal, Peyer's patches and spleen macrophages' phagocytic activity in healthy mice and monocyte chemotactic protein (MCP-1) released by intestinal epithelial cells in an in vitro assay. Although Lact. acidophilus increased the number of IgA-secreting cells previous and postinfection, and Lact. bulgaricus increased MCP-1 released by intestinal epithelial cells and the phagocytic activity of macrophages, these effects alone were not enough to confer protection against Salmonella Typhimurium infection in mouse.
Probiotic strain Lact. casei CRL 431 was the one that induced protection against Salmonella, by increasing the intestinal barrier function and by decreasing the local inflammatory response.
Significance and Impact of the Study
Salmonella spp. constitutes an important agent of foodborne diseases in the world. Not all lactobacilli, even with some immunostimulating properties at gut level, can protect against Salmonella infection. Lactobacillus casei CRL 431, a probiotic bacterium, could be useful as an oral mucosal adjuvant of the immune system to improve gut health, especially in the prevention or amelioration of Salmonella infections. We demonstrated that there is not a unique mechanism by which this protective effect was exerted.