Responses of community structure of amoA-encoding archaea and ammonia-oxidizing bacteria in ammonia biofilter with rockwool mixtures to the gradual increases in ammonium and nitrate
Article first published online: 27 DEC 2012
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 3, pages 746–761, March 2013
How to Cite
Yasuda, T., Waki, M., Kuroda, K., Hanajima, D., Fukumoto, Y., Yamagishi, T., Suwa, Y. and Suzuki, K. (2013), Responses of community structure of amoA-encoding archaea and ammonia-oxidizing bacteria in ammonia biofilter with rockwool mixtures to the gradual increases in ammonium and nitrate. Journal of Applied Microbiology, 114: 746–761. doi: 10.1111/jam.12091
- Issue published online: 18 FEB 2013
- Article first published online: 27 DEC 2012
- Accepted manuscript online: 1 DEC 2012 01:27AM EST
- Manuscript Accepted: 22 NOV 2012
- Manuscript Revised: 21 NOV 2012
- Manuscript Received: 10 SEP 2012
- Ministry of Agriculture, Forestry and Fisheries of Japan
- ammonia-oxidizing bacteria;
- amoA-encoding archaea;
- community responses;
- free ammonia;
- laboratory-scale rockwool biofilter;
- nitrogen mass balance
To investigate community shifts of amoA-encoding archaea (AEA) and ammonia-oxidizing bacteria (AOB) in biofilter under nitrogen accumulation process.
Methods and Results
A laboratory-scale rockwool biofilter with an irrigated water circulation system was operated for 436 days with ammonia loading rates of 49–63 NH3 g m−3 day−1. The AEA and AOB communities were investigated by denaturing gradient gel electrophoresis, sequencing and real-time PCR analysis based on amoA genes. The results indicated that changes in abundance and community compositions occurred in a different manner between archaeal and bacterial amoA during the operation. However, both microbial community structures mainly varied when free ammonia (FA) concentrations in circulation water were increasing, which caused a temporal decline in reactor performance. Dominant amoA sequences after this transition were related to Thaumarchaeotal Group I.1b, Nitrosomonas europaea lineages and one subcluster within Nitrosospira sp. cluster 3, for archaea and bacteria, respectively.
The specific FA in circulation water seems to be the important factor, which relates to the AOB and AEA community shifts in the biofilter besides ammonium and pH.
Significance and Impact of the Study
One of the key factors for regulating AEA and AOB communities was proposed that is useful for optimizing biofiltration technology.