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Figure S1 29N2 (1) and 30N2 (2) production during the anaerobic incubation of the packing materials sampled on day 150 (a) and day 317 (b) with NH4+ and 15NO3 in the tracer experiment. White and grey symbols represent the two replications.

Figure S2 29N2 production during the anaerobic incubation of the packing materials sampled on day 150 (a) and day 317 (b) with 15NH4+ and NO2 in the tracer experiment.

Figure S3 (a) DGGE patterns of archael amoA genes amplified with CrenamoA23f/crenamoA616r. Numbers above the band profiles indicate the sampling days; from the packing materials of the reactor on days 0, 32, 150, 317, 436, and from the circulation water on days 317 and 436. (b) Principal component analysis of DGGE bands of Fig. S3 (a).

Table S1 The importance probability (Pi) of DGGE bands and Shannon index (H) of each sample from DGGE profiles.

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