Characterization of food spoilage fungi by FTIR spectroscopy
Article first published online: 7 JAN 2013
© 2012 Nofima AS © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 3, pages 788–796, March 2013
How to Cite
Shapaval, V., Schmitt, J., Møretrø, T., Suso, H.P., Skaar, I., Åsli, A.W., Lillehaug, D. and Kohler, A. (2013), Characterization of food spoilage fungi by FTIR spectroscopy. Journal of Applied Microbiology, 114: 788–796. doi: 10.1111/jam.12092
- Issue published online: 18 FEB 2013
- Article first published online: 7 JAN 2013
- Accepted manuscript online: 4 DEC 2012 11:45AM EST
- Manuscript Accepted: 10 NOV 2012
- Manuscript Revised: 22 OCT 2012
- Manuscript Received: 19 MAR 2012
- Agricultural Food Research Foundation of Norway
- Research Council of Norway
- food spoilage;
- FTIR spectroscopy;
The objective of the study was to evaluate a high-throughput liquid microcultivation protocol and FTIR spectroscopy for the differentiation of food spoilage filamentous fungi.
Methods and Results
For this study, fifty-nine food-related fungal strains were analysed. The cultivation of fungi was performed in liquid medium in the Bioscreen C microtitre plate system with a throughput of 200 samples per cultivation run. Mycelium was prepared for FTIR analysis by a simple procedure, including a washing and a homogenization step. Hierarchical cluster analysis was used to study affinity among the different species. Based on the hierarchical cluster analysis, a classification and validation scheme was developed by artificial neural network analysis. The classification network was tested by an independent test set. The results show that 93·9 and 94·0% of the spectra were correctly identified at the species and genus level, respectively.
The use of high-throughput liquid microcultivation protocol combined with FTIR spectroscopy and artificial neural network analysis allows differentiation of food spoilage fungi on the phylum, genus and species level.
Significance and Impact of the Study
The high-throughput liquid microcultivation protocol combined with FTIR spectroscopy can be used for the detection, classification and even identification of food-related filamentous fungi. Advantages of the method are high-throughput characteristics, high sensitivity, low costs and relatively short time of analysis.