Bacillus sp. SXB and Pantoea sp. IMH, aerobic As(V)-reducing bacteria isolated from arsenic-contaminated soil
Article first published online: 28 DEC 2012
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 3, pages 713–721, March 2013
How to Cite
Wu, Q., Du, J., Zhuang, G. and Jing, C. (2013), Bacillus sp. SXB and Pantoea sp. IMH, aerobic As(V)-reducing bacteria isolated from arsenic-contaminated soil. Journal of Applied Microbiology, 114: 713–721. doi: 10.1111/jam.12093
- Issue published online: 18 FEB 2013
- Article first published online: 28 DEC 2012
- Accepted manuscript online: 4 DEC 2012 11:46AM EST
- Manuscript Accepted: 30 NOV 2012
- Manuscript Revised: 29 NOV 2012
- Manuscript Received: 28 SEP 2012
- National Natural Science Foundation of China. Grant Numbers: 20977098, 20890112, 20921063
- ars genes;
- As-contaminated soil;
To isolate highly effective aerobic As(V)-reducing bacteria from arsenic(As)-contaminated soils in Northwest China and to identify their dynamic As(V) reduction processes and genomic detoxification mechanisms.
Methods and Results
Enrichment cultures were performed aerobically in tryptone, yeast extract and glucose medium in the presence of As(V). Strain SXB isolated from soil in Shanxi Province, belonging to Bacillus genus, reduced As(V) more effectively under aerobic conditions than under anaerobic conditions. Strain IMH, a strictly aerobic isolate obtained from soil in Inner Mongolia, identified as Pantoea, is reported for the first time to reduce As(V). Both isolates could reduce over 90% As(V) in 36 h under aerobic conditions. Putative gene fragments for the ArsB efflux pump gene were obtained from both strains. The putative arsenate reductase gene was only amplified from strain SXB. A putative arsH gene was amplified from strain IMH.
Strains SXB and IMH isolated from the As-contaminated soils reduce As(V) effectively under aerobic conditions via a detoxification mechanism regulated by ars operons.
Significance and Impact of the Study
Pantoea genus is reported to reduce As(V) for the first time. This study provides a full understanding of the highly effective As(V)-reducing bacteria SXB and IMH, which could influence the As biogeochemical cycle in soils.