Silver colloidal nanoparticles: effect on matrix composition and structure of Candida albicans and Candida glabrata biofilms
Article first published online: 11 JAN 2013
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 4, pages 1175–1183, April 2013
How to Cite
Monteiro, D.R., Silva, S., Negri, M., Gorup, L.F., de Camargo, E.R., Oliveira, R., Barbosa, D.B. and Henriques, M. (2013), Silver colloidal nanoparticles: effect on matrix composition and structure of Candida albicans and Candida glabrata biofilms. Journal of Applied Microbiology, 114: 1175–1183. doi: 10.1111/jam.12102
- Issue published online: 18 MAR 2013
- Article first published online: 11 JAN 2013
- Accepted manuscript online: 12 DEC 2012 06:33AM EST
- Manuscript Accepted: 5 DEC 2012
- Manuscript Revised: 13 NOV 2012
- Manuscript Received: 26 JUL 2012
- FAPESP. Grant Number: BEX 1221/10-8
- CAPES. Grant Numbers: BEX 1221/10-8, 2009/15146-5
- Candida albicans ;
- Candida glabrata ;
- fungal infections;
- silver nanoparticles
The aim of this study was to assess the effect of different silver nanoparticles (SN) concentrations on the matrix composition and structure of Candida albicans and Candida glabrata biofilms.
Methods and Results
Candida biofilms were developed in 6-well microtiter plates during 48 h. After, these biofilms were exposed to 13·5 or 54 μg SN ml−1 for 24 h. Then, extracellular matrices were extracted from biofilms and analysed chemically in terms of proteins, carbohydrates and DNA. To investigate the biofilm structure, scanning electron microscopy (SEM) and epifluorescence microscopy were used. SN interfered with the matrix composition of Candida biofilms tested in terms of protein, carbohydrate and DNA, except for the protein content of C. albicans biofilm. By SEM, Candida biofilms treated with SN revealed structural differences, when compared with the control groups. Further, SN showed a trend of agglomeration within the biofilms. Epifluorescence microscopy images suggest that SN induced damage on cell walls of the Candida isolates tested.
In general, irrespective of concentration, SN affected the matrix composition and structure of Candida biofilms and these findings may be related to the mechanisms of biocide action of SN.
Significance and Impact of the Study
This study reveals new insights about the behaviour of SN when in contact with Candida biofilms. SN may contribute to the development of therapies to prevent or control Candida infections.