Selective carbohydrate utilization by lactobacilli and bifidobacteria
Article first published online: 11 JAN 2013
© 2012 University College Cork © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 4, pages 1132–1146, April 2013
How to Cite
Watson, D., O'Connell Motherway, M., Schoterman, M.H.C., van Neerven, R.J. J., Nauta, A. and van Sinderen, D. (2013), Selective carbohydrate utilization by lactobacilli and bifidobacteria. Journal of Applied Microbiology, 114: 1132–1146. doi: 10.1111/jam.12105
- Issue published online: 18 MAR 2013
- Article first published online: 11 JAN 2013
- Accepted manuscript online: 15 DEC 2012 06:56AM EST
- Manuscript Revised: 10 DEC 2012
- Manuscript Accepted: 10 DEC 2012
- Manuscript Received: 7 AUG 2012
- FrieslandCampina. Grant Number: PDTM/20011/9
- Science Foundation Ireland (SFI)
- Irish Government's National Development Plan. Grant Number: 02/CE/B124 and 07/CE/B1368
- Bifidobacterium ;
- Lactobacillus ;
To evaluate the ability of specific carbohydrates, including commercially available products, to support the growth of representatives of two well-known groups of gut commensals, namely lactobacilli and bifidobacteria.
Methods and Results
Sixty-eight bacterial strains, representing 29 human-derived lactobacilli and 39 bifidobacteria (both human- and animal-derived), were tested for their ability to metabolize 10 different carbohydrates. Analysis of growth and metabolic activity was performed using a combination of diagnostic parameters, such as final OD600, final pH, fermentation end products and growth rate.
The data assembled in this study provide significant complementary and comparative information on the growth-promoting properties of a range of carbohydrates, while also investigating interspecies differences between lactobacilli and/or bifidobacteria with regard to their carbohydrate utilization abilities. Galacto-oligosaccharides (GOS) and lactulose were shown to support the most favourable growth characteristics, whereas relatively poor growth of lactobacilli and bifidobacteria was observed on inulin, maltodextrin and polydextrose. GOS/inulin (9 : 1) and fructo-oligosaccharides (FOS)/inulin mixtures supported mostly similar growth abilities to those obtained for GOS and FOS, respectively. Microbial consumption of GOS, as determined by high-performance anion-exchange chromatography with pulsed amperometric detection, was evident for both lactobacilli and bifidobacteria.
Significance and Impact of the Study
These results may allow for the rational prediction of lactobacilli and/or bifidobacteria to be used in conjunction with prebiotics, such as GOS, as synbiotics.