Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis
Article first published online: 31 JAN 2013
© 2012 The Society for Applied Microbiology No claim to US Government works
Journal of Applied Microbiology
Volume 114, Issue 4, pages 992–1000, April 2013
How to Cite
Seiner, D.R., Colburn, H.A., Baird, C., Bartholomew, R.A., Straub, T., Victry, K., Hutchison, J.R., Valentine, N. and Bruckner-Lea, C.J. (2013), Evaluation of the FilmArray® system for detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis. Journal of Applied Microbiology, 114: 992–1000. doi: 10.1111/jam.12107
- Issue published online: 18 MAR 2013
- Article first published online: 31 JAN 2013
- Accepted manuscript online: 27 DEC 2012 08:57AM EST
- Manuscript Accepted: 17 DEC 2012
- Manuscript Revised: 10 DEC 2012
- Manuscript Received: 28 MAY 2012
- biothreat panel, FilmArray®;
- first responders;
- hand portable;
- PCR ;
- sample preparation
To evaluate the sensitivity and specificity of the BioFire Diagnostics FilmArray® system in combination with their Biothreat Panel for the detection of Bacillus anthracis (Ba), Francisella tularensis (Ft) and Yersinia pestis (Yp) DNA, and demonstrate the detection of Ba spores.
Methods and Results
DNA samples from Ba, Ft and Yp strains and near-neighbours, and live Ba spores were analysed using the FilmArray® Biothreat Panel, a multiplexed PCR-based assay for 17 pathogens and toxins. Sensitivity studies with DNA indicate that the limit of detection is 250 genome equivalents (GEs) per sample or lower. Furthermore, the identification of Ft, Yp or Bacillus species was made in 63 of 72 samples tested at 25 GE or less. With samples containing 25 CFU of Ba Sterne spores, at least one of the two possible Ba markers was identified in all samples tested. We observed no cross-reactivity with near-neighbour DNAs.
Our results indicate that the FilmArray® Biothreat Panel is a sensitive and selective assay for detecting the genetic signatures of Ba, Ft and Yp.
Significance and Impact of the Study
The FilmArray® platform is a complete sample-to-answer system, combining sample preparation, PCR and data analysis. This system is particularly suited for biothreat testing where samples need to be analysed for multiple biothreats by operators with limited training.