Utilization and optimization of a waste stream cellulose culture medium for pigment production by Penicillium spp.
Article first published online: 29 JAN 2013
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 3, pages 733–745, March 2013
How to Cite
Sopandi, T., Wardah, A., Surtiningsih, T., Suwandi, A. and Smith, J.J. (2013), Utilization and optimization of a waste stream cellulose culture medium for pigment production by Penicillium spp. Journal of Applied Microbiology, 114: 733–745. doi: 10.1111/jam.12110
- Issue published online: 18 FEB 2013
- Article first published online: 29 JAN 2013
- Accepted manuscript online: 26 DEC 2012 07:32AM EST
- Manuscript Accepted: 19 NOV 2012
- Manuscript Revised: 12 NOV 2012
- Manuscript Received: 8 JUL 2012
- Directorate General of Higher Education, National Education Department of Indonesia
- Penicillium resticulosum ;
- waste stream reuse
This research sought to determine optimal corn waste stream–based fermentation medium C and N sources and incubation time to maximize pigment production by an indigenous Indonesian Penicillium spp., as well as to assess pigment pH stability.
Methods and Results
A Penicillium spp. was isolated from Indonesian soil, identified as Penicillium resticulosum, and used to test the effects of carbon and nitrogen type and concentrations, medium pH, incubation period and furfural on biomass and pigment yield (PY) in a waste corncob hydrolysate basal medium. Maximum red PY (497·03 ± 55·13 mg l−1) was obtained with a 21 : 1 C : N ratio, pH 5·5–6·0; yeast extract-, NH4NO3-, NaNO3-, MgSO4·7H2O-, xylose- or carboxymethylcellulose (CMC)-supplemented medium and 12 days (25°C, 60–70% relative humidity, dark) incubation. C source, C, N and furfural concentration, medium pH and incubation period all influenced biomass and PY. Pigment was pH 2–9 stable.
Penicillium resticulosum demonstrated microbial pH-stable-pigment production potential using a xylose or CMC and N source, supplemented waste stream cellulose culture medium.
Significance and Impact of the Study
Corn derived, waste stream cellulose can be used as a culture medium for fungal pigment production. Such application provides a process for agricultural waste stream resource reuse for production of compounds in increasing demand.