Shiga toxin-producing Escherichia coli in drinking water supplies of north Paraná State, Brazil
Article first published online: 14 JAN 2013
© 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 4, pages 1230–1239, April 2013
How to Cite
Lascowski, K.M.S., Guth, B.E.C., Martins, F.H., Rocha, S.P.D., Irino, K. and Pelayo, J.S. (2013), Shiga toxin-producing Escherichia coli in drinking water supplies of north Paraná State, Brazil. Journal of Applied Microbiology, 114: 1230–1239. doi: 10.1111/jam.12113
- Issue published online: 18 MAR 2013
- Article first published online: 14 JAN 2013
- Accepted manuscript online: 26 DEC 2012 10:26AM EST
- Manuscript Accepted: 18 DEC 2012
- Manuscript Revised: 7 DEC 2012
- Manuscript Received: 13 AUG 2012
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- drinking water;
- pulsed-field gel electrophoresis;
- Shiga toxin-producing Escherichia coli;
- virulence markers
To determine the occurrence and characteristics of Shiga toxin-producing Escherichia coli (STEC) in drinking water supplies treated and untreated.
Methods and Results
Drinking water samples (n = 1850) were collected from 41 municipalities in the north of Paraná State between February 2005 and January 2006. Escherichia coli isolates (n = 300) were recovered from water and investigated for the presence of virulence markers related to STEC by PCR. STEC isolates recovered were then characterized for both phenotypic and genotypic traits. A total of 12 isolates (11 from untreated water and one from treated water) were positive for stx, including five positive for both stx1 and stx2, two positive for stx1 and five positive for stx2. None of the STEC isolates contained eae, but other virulence genes were observed such as ehxA (100%), saa (100%), lpfAO113 (75%), iha (42%), subAB (25%) and cdtV (8%). Multidrug resistance was identified in 25% of the STEC isolates. The 12 STEC isolates belonged to seven distinct serotypes and pulsed-field gel electrophoresis typing revealed the presence of two clusters and two clones in this region.
Drinking water, especially from untreated water supplies, can be source of STEC strains potentially pathogenic for humans.
Significance and Impact of the Study
The investigation of the drinking water supplies for pathogenic E. coli, as STEC, may be useful to prevent waterborne outbreaks.