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Evaluating glucose and xylose as cosubstrates for lipid accumulation and γ-linolenic acid biosynthesis of Thamnidium elegans

Authors


Correspondence

Seraphim Papanikolaou, Laboratory of Food Microbiology and Biotechnology, Department of Food Science and Technology, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece. E-mail: spapanik@aua.gr

Abstract

Aims

To study the biotechnological production of lipids containing rich amounts of the medically and nutritionally important γ-linolenic acid (GLA), during cultivation of the Zygomycetes Thamnidium elegans, on mixtures of glucose and xylose, abundant sugars of lignocellulosic biomass.

Methods and Results

Glucose and xylose were utilized as carbon sources, solely or in mixtures, under nitrogen-limited conditions, in batch-flask or bioreactor cultures. On glucose, T. elegans produced 31·9 g l−1 of biomass containing 15·0 g l−1 lipid with significantly high GLA content (1014 mg l−1). Xylose was proved to be an adequate substrate for growth and lipid production. Additionally, xylitol secretion occurred when xylose was utilized as carbon source, solely or in mixtures with glucose. Batch-bioreactor trials on glucose yielded satisfactory lipid production, with rapid substrate consumption rates. Analysis of intracellular lipids showed that the highest GLA content was observed in early stationary growth phase, while the phospholipid fraction was the most unsaturated fraction of T. elegans.

Conclusions

Thamnidium elegans represents a promising fungus for the successful valorization of sugar-based lignocellulosic residues into microbial lipids of high nutritional and pharmaceutical interest.

Significance and Impact of the Study

Xylitol production and cultivation in bioreactor trials is reported for the first time for T. elegans, while cultivation on xylose-based media resulted in high GLA production by this fungus.

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