Evaluation of a IS6110-Taqman real-time PCR assay to detect Mycobacterium tuberculosis in sputum samples of patients with pulmonary TB
Article first published online: 11 JAN 2013
© Aggeu Magalhães Research Center © 2012 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 4, pages 1103–1108, April 2013
How to Cite
Lira, L.A.S., Santos, F.C.F., Carvalho, M.S.Z., Montenegro, R.A., Lima, J.F.C., Schindler, H.C. and Montenegro, L.M.L. (2013), Evaluation of a IS6110-Taqman real-time PCR assay to detect Mycobacterium tuberculosis in sputum samples of patients with pulmonary TB. Journal of Applied Microbiology, 114: 1103–1108. doi: 10.1111/jam.12119
- Issue published online: 18 MAR 2013
- Article first published online: 11 JAN 2013
- Accepted manuscript online: 26 DEC 2012 10:29AM EST
- Manuscript Accepted: 20 DEC 2012
- Manuscript Revised: 19 DEC 2012
- Manuscript Received: 17 OCT 2012
- Mycobacterium tuberculosis ;
Evaluate the IS6110-Taqman system performance in sputum samples from patients with pulmonary tuberculosis from health services in north-eastern Brazil as a diagnostic laboratory tool for pulmonary tuberculosis.
Methods and Results
165 sputum samples from respiratory symptomatic patients were evaluated in the IS6110-TaqMan assay: 66 patients with pulmonary tuberculosis and 99 without TB. When the IS6110-TaqMan assay was evaluated using culture and/or clinical response to the specific treatment as the gold standard, IS6110-TaqMan assay obtained a sensitivity of 87·9% and specificity of 98%. The performance of IS6110-TaqMan assay was also evaluated with the sputum smear microscopy, resulting in a sensitivity of 79·7% and specificity 94·8%.
The IS6110-TaqMan was rapid, sensitive and specific for the diagnosis of pulmonary TB.
Significance and Impact of the Study
IS6110-TaqMan assay is a promising auxiliary tool for the diagnosis of pulmonary TB when used in conjunction with routine laboratory tests, clinical and epidemiological criteria of the patient, thus increasing the sensitivity and specificity of diagnosis.