Absence of tetracycline resistance in Campylobacter coli isolates from Finnish finishing pigs treated with chlortetracycline
Article first published online: 1 FEB 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 4, pages 974–981, April 2013
How to Cite
Juntunen, P., Laurila, T., Heinonen, M. and Hänninen, M.-L. (2013), Absence of tetracycline resistance in Campylobacter coli isolates from Finnish finishing pigs treated with chlortetracycline. Journal of Applied Microbiology, 114: 974–981. doi: 10.1111/jam.12120
- Issue published online: 18 MAR 2013
- Article first published online: 1 FEB 2013
- Accepted manuscript online: 2 JAN 2013 11:20AM EST
- Manuscript Accepted: 19 DEC 2012
- Manuscript Revised: 7 DEC 2012
- Manuscript Received: 12 OCT 2012
- Walter Ehrström Foundation. Grant Number: 141140
- Graduate School of the Veterinary Faculty
- antimicrobial susceptibility;
- Campylobacter coli ;
- Enterococcus ;
- minimum inhibitory concentrations;
To determine whether therapeutic treatment of pigs with chlortetracycline affects the susceptibility of their Campylobacter isolates for tetracycline, ciprofloxacin and erythromycin.
Methods and Results
Minimum inhibitory concentrations (MICs) and presence of a tetracycline resistance gene tet(O) were studied in Campylobacter collected before, during and after chlortetracycline treatment. Tetracycline MICs and the presence of tet(O) for additional Campylobacter coli isolates collected previously from seven farrowing farms were also determined. Isolates with ciprofloxacin MICs above the epidemiological cut-off value (ECOFF) were subtyped by flaA restriction fragment length polymorphism (RFLP). Tetracycline MICs of 221 Camp. coli isolates remained under the ECOFF at all sampling stages as well as the MICs for 63 isolates from the other farms. The ciprofloxacin MIC was above the ECOFF for 22% of the isolates, and one Camp. coli isolate had an erythromycin MIC above the ECOFF. None of the studied 300 Campylobacter isolates from nine herds carried tet(O). flaA-RFLP typing revealed the heterogeneity of Camp. coli isolates with high ciprofloxacin MICs.
Use of chlortetracycline did not increase the MIC values for the antimicrobials studied.
Significance and Impact of the Study
This study demonstrated that susceptibility of Camp. coli isolates is not affected by chlortetracycline therapy if tet(O) is not present in Camp. coli population.