Radiorespirometric assays for the detection of biogenic sulfides from sulfate-reducing bacteria
Article first published online: 29 JAN 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 4, pages 1008–1019, April 2013
How to Cite
de Queiroz, J.C., de Melo Ferreira, A.C. and da Costa, A.C.A. (2013), Radiorespirometric assays for the detection of biogenic sulfides from sulfate-reducing bacteria. Journal of Applied Microbiology, 114: 1008–1019. doi: 10.1111/jam.12122
- Issue published online: 18 MAR 2013
- Article first published online: 29 JAN 2013
- Accepted manuscript online: 5 JAN 2013 02:40AM EST
- Manuscript Accepted: 31 DEC 2012
- Manuscript Revised: 22 DEC 2012
- Manuscript Received: 8 OCT 2012
- culture medium;
- most probable number;
- sulfate-reducing bacteria;
The detection of trace concentrations of biogenic sulfides can be carried out through radiorespirometric assays. The objective of this work was to improve the methodology for detection of H2S in trace concentrations, to correlate with sulfate-reducing bacterial activity.
Methods and Results
Serial dilutions of synthetic sea water with a pure culture of Desulfovibrio alaskensis, a mixed anaerobic microbial culture and a natural saline sample from a petroleum offshore platform indicated that dilutions were followed, accordingly, by sulfate reduction.
Tests performed indicated that increasing the time of incubation of a mixed anaerobic microbial culture contributed to an increase in the sulfate reduction rates, as well as the amount of carbon source and inoculum.
Significance and Impact of the Study
The technique here developed proved to be a rapid test for the detection of biogenic sulfides, particularly those associated with corrosion products, being an useful tool for monitoring and controlling oil/water storage tanks, petroleum continental platforms and several types of reservoirs.