Assessment of polycyclic aromatic hydrocarbon biodegradation potential in mangrove sediment from Don Hoi Lot, Samut Songkram Province, Thailand
Article first published online: 1 FEB 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 5, pages 1311–1324, May 2013
How to Cite
Muangchinda, C., Pansri, R., Wongwongsee, W. and Pinyakong, O. (2013), Assessment of polycyclic aromatic hydrocarbon biodegradation potential in mangrove sediment from Don Hoi Lot, Samut Songkram Province, Thailand. Journal of Applied Microbiology, 114: 1311–1324. doi: 10.1111/jam.12128
- Issue published online: 15 APR 2013
- Article first published online: 1 FEB 2013
- Accepted manuscript online: 7 JAN 2013 01:16PM EST
- Manuscript Accepted: 2 JAN 2013
- Manuscript Revised: 1 JAN 2013
- Manuscript Received: 30 OCT 2012
- Thai Government Stimulus Package 2. Grant Number: TKK2555
- denaturing gradient gel electrophoresis;
To assess the biodegradation potential of mixed polycyclic aromatic hydrocarbons (PAHs) in mangrove sediments.
Methods and Results
Sediment microcosms were constructed with sediment collected from Don Hoi Lot, Samut Songkram Province, Thailand, by supplementation with a mixture of acenaphthene, phenanthrene and pyrene. At the end of 8 weeks, low molecular weight PAHs, acenaphthene and phenanthrene were completely degraded. PCR-denaturing gradient gel electrophoresis profile suggests that Marinobacter, Enterobacter and Dethiosulfatibacter play important roles in PAH degradation in mangrove sediment. Furthermore, six PAH-degrading bacteria were isolated consisting of novel phenanthrene-degrading Dyella sp. and Luteibacter sp., phenanthrene-degrading Burkholderia sp., acenaphthene-degrading Alcaligenes sp. and pyrene-degrading Ochrobactrum sp. Moreover, dioxygenase genes could be detected both in sediment microcosms as well as in all of the isolated strains.
These results demonstrated that indigenous bacteria in the mangrove sediment had the ability to degrade phenanthrene in the presence of mixture PAHs with high efficacy.
Significance and Impact of the Study
Culture and nonculture methods were combined to assess PAH biodegradation in mangrove sediment. Two novel phenanthrene-degrading bacteria were isolated. Three genera of bacteria that play important roles in PAH degradation were indicated by nonculture approach. Moreover, dioxygenase genes could be detected. This information is useful for further bioremediation of PAH-contaminated mangrove sediments.