Neuraminidase Inhibitors from marine-derived actinomycete Streptomyces seoulensis
Article first published online: 8 FEB 2013
© 2013 Institute of Functional Biomolecules © 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 4, pages 1046–1053, April 2013
How to Cite
Jiao, R.H., Xu, H., Cui, J.T., Ge, H.M. and Tan, R.X. (2013), Neuraminidase Inhibitors from marine-derived actinomycete Streptomyces seoulensis. Journal of Applied Microbiology, 114: 1046–1053. doi: 10.1111/jam.12136
- Issue published online: 18 MAR 2013
- Article first published online: 8 FEB 2013
- Accepted manuscript online: 13 JAN 2013 02:25AM EST
- Manuscript Accepted: 7 JAN 2013
- Manuscript Revised: 26 DEC 2012
- Manuscript Received: 10 NOV 2012
- National Natural Science Foundation of China. Grant Numbers: 21132004, 30901846, 81121062
- The Ministry of Science & Technology. Grant Numbers: 2011AA09070204, 2013AA092901
- marine-derived actinomycete;
- neuraminidase inhibitors;
- Streptomyces seoulensis
This work was performed to characterize new secondary metabolites with neuraminidase (NA) inhibitory activity from marine actinomycete strains.
Methods and Results
An actinomycete strain IFB-A01, capable of producing new NA inhibitors, was isolated from the gut of shrimp Penasus orientalis and identified as Streptomyces seoulensis according to its 16S rRNA sequence (over 99% homology with that of the standard strain). Repeated chromatography of the methanol extract of the solid-substrate culture of S. seoulensis IFB-A01 led to the isolation of streptoseolactone (1), and limazepines G (2) and H (3). The structures of 1–3 were determined by a combination of IR, ESI-MS, 1D (1H and 13C NMR, and DEPT) and 2D NMR experiments (HMQC, HMBC, 1H-1H COSY and NOESY). Compounds 1–3 showed significant inhibition on NA in a dose-dependent manner with IC50 values of 3·92, 7·50 and 7·37 μmol l−1, respectively.
This is the first report of two new (1 and 2) and known (3, recovered as a natural product for the first time in the work) NA inhibitors from the marine-derived actinomycete S. seoulensis IFB-A01.
Significance and Impact of the Study
The three natural NA inhibitors maybe of value for the development of drug(s) necessitated for the treatment of viral infections.