These authors contributed equally to this work.
Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1
Article first published online: 13 MAR 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 5, pages 1338–1346, May 2013
How to Cite
Armando, M.R., Galvagno, M.A., Dogi, C.A., Cerrutti, P., Dalcero, A.M. and Cavaglieri, L.R. (2013), Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1. Journal of Applied Microbiology, 114: 1338–1346. doi: 10.1111/jam.12144
- Issue published online: 15 APR 2013
- Article first published online: 13 MAR 2013
- Accepted manuscript online: 24 JAN 2013 08:25AM EST
- Manuscript Accepted: 28 NOV 2012
- Manuscript Revised: 13 NOV 2012
- Manuscript Received: 9 SEP 2012
- biomass production;
- cane molasses;
- fumonisin B1;
- Saccharomyces cerevisiae ;
- statistical optimization
To evaluate the ability of probiotic Saccharomyces cerevisiae RC016 strain to reduce fumonisin B1 (FB1) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology.
Methods and Results
Using Plackett–Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (g l−1) fermentable sugars provided by sugar cane molasses (CMs), yeast extract (YE) and (NH4)2HPO4 (DAP) was formulated. The S. cerevisiae RC016 strain showed the greatest binding at all assayed FB1 concentration. The CMs, YE, DAP concentrations and incubation time influenced significantly the biomass of S. cerevisiae RC016.
A combination of CMs 17%; YE 4·61 g l−1 and incubation time 60 h was optimum for maximum biomass of S. cerevisiae RC016.
Significance and Impact of the Study
The importance of this work lies in the search for live strains with both probiotic and fumonisin B1 decontamination properties that could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources and would be included in a novel product to animal feed.