Bacteriostatic effect of sequential hydrodynamic and ultrasound-induced stress
Article first published online: 13 FEB 2013
© 2013 Ashland Inc. © 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 4, pages 947–955, April 2013
How to Cite
Chapman, J.S., Ferguson, R., Consalo, C. and Bliss, T. (2013), Bacteriostatic effect of sequential hydrodynamic and ultrasound-induced stress. Journal of Applied Microbiology, 114: 947–955. doi: 10.1111/jam.12146
- Issue published online: 18 MAR 2013
- Article first published online: 13 FEB 2013
- Accepted manuscript online: 29 JAN 2013 11:01AM EST
- Manuscript Accepted: 18 NOV 2012
- Manuscript Revised: 12 NOV 2012
- Manuscript Received: 5 SEP 2012
- mechanism of action;
To elucidate the mechanism of action of a nonchemical microbial control technology employing coupled hydrodynamic and ultrasound-induced stress.
Methods & Results
The effects of a laboratory model system using a commercial nonchemical device on Pseudomonas putida revealed growth and respiration were inhibited without a loss of viability from the treated population. Damage to cell membranes was evident using fluorescent microscopy and a reporter strain containing lux genes fused with a membrane damage stress-response promoter. Other reporter strains also indicated the possible involvement of DNA and protein repair systems. A consequence of treatment was a reduced ability to form biofilms.
The nonchemical device caused a biostatic effect on treated cells induced by sublethal damage to several cellular systems, including cell membranes.
Significance and Impact of the Study
The study demonstrates that biostasis can be an effective mechanism for microbial control in some industrial systems and provides insight into understanding and applying this device and other nonchemical microbial control technologies to real-world problems of microbial contamination.