Reduction of aflatoxin level in aflatoxin-induced rats by the activity of probiotic Lactobacillus casei strain Shirota
Article first published online: 18 FEB 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 5, pages 1507–1515, May 2013
How to Cite
Nikbakht Nasrabadi, E., Jamaluddin, R., Abdul Mutalib, M.S., Khaza'ai, H., Khalesi, S. and Mohd Redzwan, S. (2013), Reduction of aflatoxin level in aflatoxin-induced rats by the activity of probiotic Lactobacillus casei strain Shirota. Journal of Applied Microbiology, 114: 1507–1515. doi: 10.1111/jam.12148
- Issue published online: 15 APR 2013
- Article first published online: 18 FEB 2013
- Accepted manuscript online: 25 JAN 2013 12:20PM EST
- Manuscript Revised: 17 JAN 2013
- Manuscript Accepted: 17 JAN 2013
- Manuscript Received: 22 NOV 2012
- Fundamental Research Grant Scheme (FRGS). Grant Number: 5524027
- Ministry of Higher Education of Malaysia
- aflatoxin B1;
- chronic aflatoxicosis;
- probiotic Lactobacillus casei Shirota
Aflatoxin B1 (AFB1) is considered as the most toxic food contaminant, and microorganisms, especially bacteria, have been studied for their potential to reduce the bioavailability of mycotoxins including aflatoxins. Therefore, this research investigated the efficacy of oral administration of Lactobacillus casei Shirota (LcS) in aflatoxin-induced rats.
Methods and Results
Sprague Dawley rats were divided into three groups of untreated control, the group induced with AFB1 only, and the group given probiotic in addition to AFB1. In the group induced with AFB1 only, food intake and body weight were reduced significantly. The liver and kidney enzymes were significantly enhanced in both groups induced with AFB1, but they were lower in the group given LcS. AFB1 was detected from all serum samples except for untreated control group's samples. Blood serum level of AFB1 in the group induced with AFB1 only was significantly higher than the group which received probiotic as a treatment (P < 0·05), and there was no significant difference between the control group and the group treated with probiotic.
LcS supplementation could improve the adverse effect of AFB1 induction on rats' body weight, plasma biochemical parameters and also could reduce the level of AFB1 in blood serum.
Significance and Impact of the Study
This study's outcomes contribute to better understanding of the potential of probiotic to reduce the bioavailability ofAFB1. Moreover, it can open an opportunity for future investigations to study the efficacy of oral supplementation of probiotic LcS in reducing aflatoxin level in human.