Fungal endophyte diversity in soybean
Article first published online: 4 MAR 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 5, pages 1500–1506, May 2013
How to Cite
Impullitti, A.E. and Malvick, D.K. (2013), Fungal endophyte diversity in soybean. Journal of Applied Microbiology, 114: 1500–1506. doi: 10.1111/jam.12164
- Issue published online: 15 APR 2013
- Article first published online: 4 MAR 2013
- Accepted manuscript online: 11 FEB 2013 06:27AM EST
- Manuscript Accepted: 6 FEB 2013
- Manuscript Revised: 21 JAN 2013
- Manuscript Received: 7 NOV 2012
- Doctoral Dissertation Fellowship Program
- University of Minnesota Graduate School, and the Minnesota Soybean Research and Promotion Council
To determine the identity and diversity of endophytes in soybean plants using culture-dependent (CD) and culture-independent (CI) methods.
Methods and Results
Stem samples were collected from three field-grown soybean cultivars grown to a reproductive stage in Minnesota, USA. Samples were surface disinfested, and CD and CI methods were used to assess the endophytes. For the CD method, fungi were isolated and grouped based on colony morphology, and the rDNA ITS region was sequenced to identify the cultures. The most frequently isolated genera were Cladosporium (36%), Alternaria (13%), Diaporthe (9%) and Epicoccum (9%). For the CI method, DNA was extracted from the stems, and the ITS region was amplified, cloned and sequenced for identification. The most prevalent genus detected using CI method was Cladosporium (85%).
Soybean contains a diverse array of endophytic fungi that were identified in this study. The CD method detected greater endophyte diversity (H' = 2·12) than the CI method (H' = 0·66).
Significance and Impact of the Study
The results improve our understanding of the identity and diversity of endophytic fungi that likely have different kinds of interactions with soybean plants. The results suggest that CD and CI methods should be used to study endophytes in soybean and perhaps other annual crop plants.