Pleurotus ostreatus biofilm-forming ability and ultrastructure are significantly influenced by growth medium and support type
Article first published online: 6 MAR 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 6, pages 1750–1762, June 2013
How to Cite
Pesciaroli, L., Petruccioli, M., Federici, F. and D'Annibale, A. (2013), Pleurotus ostreatus biofilm-forming ability and ultrastructure are significantly influenced by growth medium and support type. Journal of Applied Microbiology, 114: 1750–1762. doi: 10.1111/jam.12170
- Issue published online: 17 MAY 2013
- Article first published online: 6 MAR 2013
- Accepted manuscript online: 16 FEB 2013 01:21PM EST
- Manuscript Accepted: 10 FEB 2013
- Manuscript Revised: 21 JAN 2013
- Manuscript Received: 18 OCT 2012
- environmental mycology;
- microbial structure;
- Pleurotus ostreatus ;
- wastewater treatment
To investigate the effect of support and growth medium (GM) on Pleurotus ostreatus biofilm production, specific metabolic activity (SMA) and ultrastructure.
Methods and Results
Biofilms were developed on membranes covering a broad range of surface properties and, due to the applicative implications of mixed biofilms, on standard bacterial GM in stationary and shaken culture. Hydrophilic (glass fibre, Duran glass and hydroxyapatite) and mild hydrophobic (polyurethane, stainless steel, polycarbonate, nylon) supports were more adequate for biofilm attachment than the hydrophobic Teflon. Among the GM, sucrose–asparagine (SA) was more conducive to biofilm production than Luria–Bertani and M9. GM was more influential than support type on biofilm ultrastructure, and a high compactness was evident in biofilms developed on SA. Biofilms on Duran glass were more efficient than planktonic cultures in olive-mill wastewater treatment.
The main effects of support and GM variables and their binary interactions on both biofilm production and SMA were all highly significant (P < 0·001): thus, the magnitude of the effect of each variable strongly depended on the level of the other one.
Significance and Impact of the Study
There is a lack of basic information regarding physiology and ultrastructure of P. ostreatus biofilms. To our knowledge, this is the first attempt to fill this gap, thus representing a basis for future studies.