Prevalence of NDM-1 carbapenemase in patients with diarrhoea in Pakistan and evaluation of two chromogenic culture media
Article first published online: 6 MAR 2013
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Journal of Applied Microbiology
Volume 114, Issue 6, pages 1810–1816, June 2013
How to Cite
Day, K.M., Salman, M., Kazi, B., Sidjabat, H.E., Silvey, A., Lanyon, C.V., Cummings, S.P., Ali, M.N., Raza, M.W., Paterson, D.L. and Perry, J.D. (2013), Prevalence of NDM-1 carbapenemase in patients with diarrhoea in Pakistan and evaluation of two chromogenic culture media. Journal of Applied Microbiology, 114: 1810–1816. doi: 10.1111/jam.12171
- Issue published online: 17 MAY 2013
- Article first published online: 6 MAR 2013
- Accepted manuscript online: 19 FEB 2013 12:49PM EST
- Manuscript Accepted: 12 FEB 2013
- Manuscript Revised: 18 JAN 2013
- Manuscript Received: 22 NOV 2012
To evaluate two chromogenic media, Brilliance CRE and chromID CARBA, with stool samples referred to the Public Health Laboratories Division of the National Institute of Health in Islamabad, and assess the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in this population.
Methods and Results
One hundred and fifty-two stool samples from patients with diarrhoea were referred to the Microbiology Department and were investigated for the presence of CPE using two chromogenic culture media, Brilliance CRE and chromID CARBA. Thirteen patients (8·6%) were found to be colonized with CPE and all produced NDM-1 carbapenemase. Twelve of these patients (92%) were found to be colonized by culture on chromID CARBA compared with seven (54%) using Brilliance CRE.
If only coloured colonies were considered as presumptive CPE, the sensitivity, specificity and positive predictive value were 54, 23 and 6% for Brilliance CRE and 85, 85 and 36% for chromID CARBA, respectively.
Significance and Impact of the Study
We conclude that Enterobacteriaceae that produce NDM-1 carbapenemase can be found in patients from all major provinces of Pakistan and that chromID CARBA was the most effective of the two chromogenic media in this setting.