Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing

Authors

  • A. Tall,

    1. Laboratoire Microbiologie-LNR, Unité Environnement, Microbiologie et Phycotoxines, Département Ressources Biologiques et Environnement, Centre de Brest, Ifremer, Plouzané, France
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  • D. Hervio-Heath,

    1. Laboratoire Microbiologie-LNR, Unité Environnement, Microbiologie et Phycotoxines, Département Ressources Biologiques et Environnement, Centre de Brest, Ifremer, Plouzané, France
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  • A. Teillon,

    1. Laboratoire Microbiologie-LNR, Unité Environnement, Microbiologie et Phycotoxines, Département Ressources Biologiques et Environnement, Centre de Brest, Ifremer, Plouzané, France
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  • C. Boisset-Helbert,

    1. Centre de Recherches sur les Macromolécules Végétales (CNRS), Plateforme de Chromatographie, Université Joseph Fourier de Grenoble, Grenoble, France
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  • R. Delesmont,

    1. Eurofins IPL Environnement, Route du Grand Colombier/Port, Gravelines, France
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  • J. Bodilis,

    1. Laboratoire de Microbiologie Signaux et Microenvironnement, Université de Rouen, Rouen, France
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  • A. Touron-Bodilis

    Corresponding author
    1. Laboratoire National d'Hydraulique et Environnement, EDF R&D, Chatou, France
    • Laboratoire Microbiologie-LNR, Unité Environnement, Microbiologie et Phycotoxines, Département Ressources Biologiques et Environnement, Centre de Brest, Ifremer, Plouzané, France
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Correspondence

Aurélie Touron-Bodilis, Laboratoire National d'Hydraulique et Environnement, EDF R&D, 6 quai Watier, 78401 Chatou cedex, France. E-mail: aurelie.touron-bodilis@edf.fr

Abstract

Aims

To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37°C on TCBS medium, in September 2009 from seawater and surface sediments.

Methods and Results

q-PCR assays previously selected for the identification of bacterial strains isolated at 37°C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22°C. The great majority of the 37°C strains was identified by q-PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85·9%) and V. harveyi (10·7%). The human pathogens V. parahaemolyticus and V. cholerae were rarely detected (two strains each). The 22°C strains were successfully identified by the phylogeny analysis of pyrH and toxR genes, revealing 20 Vibrio species, with the predominance of the clam pathogen V. celticus (36·8%). The Splendidus and the Harveyi groups represented the main Vibrio group at 22°C (80%) and 37°C (99·5%), respectively.

Conclusions

The combination of q-PCR assays and the sequencing of pyrH and toxR genes highlighted two different Vibrio communities at 22 and 37°C both dominated by pathogenic species for marine organisms.

Significance and Impact of the Study

The sequencing of the pyrH gene revealed to be a valuable tool to identify environmental Vibrio spp. strains isolated at 22°C, as 92·3% of them were identified in this study.

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