Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing
Article first published online: 26 MAR 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 6, pages 1713–1724, June 2013
How to Cite
Tall, A., Hervio-Heath, D., Teillon, A., Boisset-Helbert, C., Delesmont, R., Bodilis, J. and Touron-Bodilis, A. (2013), Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing. Journal of Applied Microbiology, 114: 1713–1724. doi: 10.1111/jam.12181
- Issue published online: 17 MAY 2013
- Article first published online: 26 MAR 2013
- Accepted manuscript online: 8 MAR 2013 07:28AM EST
- Manuscript Accepted: 25 FEB 2013
- Manuscript Revised: 15 FEB 2013
- Manuscript Received: 2 DEC 2012
- Vibrio Manche program of EDF R&D
- French Institute of research on marine exploration (Ifremer)
- Eurofins IPL Nord
- coastal environment;
- culturable Vibrio spp.;
- pyrH and toxR genes partial sequencing
To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37°C on TCBS medium, in September 2009 from seawater and surface sediments.
Methods and Results
q-PCR assays previously selected for the identification of bacterial strains isolated at 37°C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22°C. The great majority of the 37°C strains was identified by q-PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85·9%) and V. harveyi (10·7%). The human pathogens V. parahaemolyticus and V. cholerae were rarely detected (two strains each). The 22°C strains were successfully identified by the phylogeny analysis of pyrH and toxR genes, revealing 20 Vibrio species, with the predominance of the clam pathogen V. celticus (36·8%). The Splendidus and the Harveyi groups represented the main Vibrio group at 22°C (80%) and 37°C (99·5%), respectively.
The combination of q-PCR assays and the sequencing of pyrH and toxR genes highlighted two different Vibrio communities at 22 and 37°C both dominated by pathogenic species for marine organisms.
Significance and Impact of the Study
The sequencing of the pyrH gene revealed to be a valuable tool to identify environmental Vibrio spp. strains isolated at 22°C, as 92·3% of them were identified in this study.