To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37°C on TCBS medium, in September 2009 from seawater and surface sediments.
To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37°C on TCBS medium, in September 2009 from seawater and surface sediments.
q-PCR assays previously selected for the identification of bacterial strains isolated at 37°C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22°C. The great majority of the 37°C strains was identified by q-PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85·9%) and V. harveyi (10·7%). The human pathogens V. parahaemolyticus and V. cholerae were rarely detected (two strains each). The 22°C strains were successfully identified by the phylogeny analysis of pyrH and toxR genes, revealing 20 Vibrio species, with the predominance of the clam pathogen V. celticus (36·8%). The Splendidus and the Harveyi groups represented the main Vibrio group at 22°C (80%) and 37°C (99·5%), respectively.
The combination of q-PCR assays and the sequencing of pyrH and toxR genes highlighted two different Vibrio communities at 22 and 37°C both dominated by pathogenic species for marine organisms.
The sequencing of the pyrH gene revealed to be a valuable tool to identify environmental Vibrio spp. strains isolated at 22°C, as 92·3% of them were identified in this study.
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The vibrios are widespread worldwide and ubiquitous in the aquatic environments, occupying various habitats including marine, freshwater and estuarine ecosystems as well as aquaculture facilities (Dalsgaard 1998; Sobecky et al. 1998; Urakawa et al. 1999; Heidelberg et al. 2002; Thompson et al. 2004b). To date, if we refer to the various banks of genomic sequences and to the on-line sources of Vibrio species inventory (Association of Vibrio Biologists, www.vibriobiology.net, last consultation 20 August 2012), the genus Vibrio includes 135 species (Dawyndt et al. 2005). Numerous vibrios are pathogenic for marine organisms and 12 have been described as pathogenic for humans (Dalsgaard 1998; Paillard et al. 2004; Thompson et al. 2004a; Austin 2010). The sanitary and economic losses due to these pathogens, particularly to the species Vibrio cholerae, V. parahaemolyticus and V. vulnificus, have been widely described (Colwell et al. 1977; DePaola et al. 2003; Oliver 2005). In the context of global climate changes, human infections caused by vibrios are expected to increase with the increase in sea surface temperature (Tantillo et al. 2004; Baker-Austin et al. 2010; Philippart et al. 2011; Vezzulli et al. 2012). Thus, a better understanding of the natural diversity of Vibrio species and the environmental drivers of their spatial and temporal evolution are a prerequisite to address public health issues or the abnormal mortalities in hatcheries and aquaculture industries.
Today, powerful molecular techniques such as pyrosequencing or direct cell enumeration by solid or flow cytometry combined to fluorescent in situ hybridization (FISH), allow to evaluate the microbial global diversity in natural samples (Sogin et al. 2006; Alonso-Saez et al. 2007; Brown et al. 2009; Kirchman et al. 2010; Lucas et al. 2010). However, due to the low discriminatory power of 16S rRNA gene among the Vibrio genus (Thompson et al. 2004a), these methods are not applicable to an environmental study of these bacteria. In contrast, direct quantification methods like real-time PCR (q-PCR) are valuable tools to specifically detect genomic units of different Vibrio species in natural or seafood samples (Gubala and Proll 2006; Nordstrom et al. 2007). In addition to these techniques, isolation of strains remains an essential step to fully characterize the environmental strains and their potential virulence and to compare environmental monitoring and epidemiological data (mainly based on culture standards). Furthermore, establishing environmental strain collections is very useful and valuable to further study the presence and emergence of some Vibrio spp., in relation to environmental variables such as physicochemical and biological parameters.
Until the 1990s, the taxonomy of the vibrios was mainly based on morphological traits description on agar selective media and on biochemical tests. These techniques suitable for type or clinical strains are not reliable for the identification of most environmental strains due to the diversity of the metabolism expressed by these bacteria (Croci et al. 2007). The molecular biology provided decisive tools such as the PCR and DNA–DNA hybridization or more recently multilocus sequence analysis (MLSA), which improved the taxonomy within this group. Numerous phylogenetic markers, ubiquitous within the genus, were proposed for a more precise delineation of the Vibrio species (Thompson et al. 2005; Sawabe et al. 2007; Pascual et al. 2010). A MLSA comparing the species resolution level of rpoA, recA and pyrH genes, performed by Thompson et al. (2005), described the pyrH gene as a good discriminant marker at the species level. They showed a stability of this locus due to high proportions of synonymous mutations leading to the conservation of the amino acid sequence. Their work was based on a previous study from Zeigler (2003) who proposed that a single protein-encoding gene, carefully selected, could be used to assign strains to a species with an acceptable precision. He defined that the selected gene must be characterized by a wide distribution among bacteria, must be conserved long enough to be informative to have an acceptable precision in the prediction of the whole genome. Thus, the pyrH gene, fitting these criteria, constitutes a good predictor of the whole Vibrio genome and also a good discriminatory target at the species level. This has been validated by other authors for the identification of environmental strains (Chimetto et al. 2009; Alves et al. 2010; Gregoracci et al. 2012). Pascual et al. (2010) performed the same analysis on the 16S rRNA gene plus six protein-encoding genes including two of those tested by Thompson et al. (2005) (recA, pyrH, rpoD, gyrB, rctB and toxR) and determined the toxR gene as showing the most powerful taxonomic resolution within the Vibrio genus.
In a context of a 2-year study regarding the ecology of Vibrio populations and the presence of pathogenic species in French coastal waters, we previously described a strategy based on the isolation/identification of Vibrio strains at 37 and 22°C (Tall et al. 2012). We optimized q-PCR assays and we were able to identify V. alginolyticus, V. parahaemolyticus, V. vulnificus, V. cholerae and V. harveyi species. This strategy was efficient to determine the culturable diversity at 37°C as 97·2% of the strains were well identified, with V. alginolyticus, the major species, representing 85·9% of the strains. At this temperature, only 10 strains remained to be identified. However, only 2·2% of the strains isolated at 22°C were identified by q-PCR and as V. alginolyticus, leading to select the sequencing of housekeeping genes as an alternative. The present study evaluates the diversity of culturable Vibrio isolated at two temperatures of isolation (22 and 37°C) on TCBS agar medium from environmental samples in a coastal area. This was performed by combining q-PCR assays and the sequencing of the pyrH and the toxR genes of a large number of environmental Vibrio spp. strains isolated from seawater and surface sediments during the first sampling campaign (September 2009) of a 2-year study.
The study area, located in Northern France (English Channel, Pas de Calais, 59, France), has been chosen for its contrasting coastal characteristics and water uses (Fig. 1). The first subarea is located around industrial and human recreational activities (bathing, water sports and fishing) near the cities of Gravelines and Grand-Fort Philippe. The second subarea is located around a natural restricted area (Platier d'Oye). The mean seawater temperature in the area at the time of sampling (23 September and 24 September 2009) was 18·6°C (±0·2°C) and the salinity ranged from 33·5 to 34·4 ppt. Seawater and surface sediments samples were collected during an ebb tide and along two transects. The Transect 1 (T1) is located in front of the coastal segment including human and industrial activities, and the Transect 2 (T2) is located in front of the natural restricted area. To study the coast-off distribution of Vibrio abundance and diversity, at the two temperatures of isolation, water and sediment samples were collected along both transects including four different sampling points, one at the coast and the three others at 300, 1300 and 3000 m from the coast with mean depths of 4, 6, 9 and 23 m, respectively (Fig. 1). To study a depth gradient, surface and bottom seawater and surface sediments were sampled at each point (except for the first point at the coast, only surface water was sampled). Two litres of surface and bottom seawater were sampled at each point using a Van Dorn bottle and conditioned into two-litre sterile flasks to perform bacteriological analyses. The surface sediments were sampled at each point using a metallic grab sampler and conditioned into one-litre sterile flasks. The samples (14 sea water samples and 8 sediment samples) were transported in isothermal condition to the laboratory for bacterial strains isolation and analysed within 5–6 h.
The isolation and conservation of the Vibrio strains were performed according to Tall et al. (2012). Briefly, seawater samples were filtered (0·1, 1, 10, 50 and 100 ml) onto 0·45-μm-pore-size nitrocellulose filters (Millipore™ SA, ST Quentin Les Yvelines, France). Ten grams of sediment samples were diluted in 90 ml of tryptone salt, mixed during 10 min and filtered (10–25 ml) through similar membranes or the dilutions were directly plated. Membranes and dilutions were plated onto thiosulphate–citrate–bile salts–sucrose agar plates (TCBS, Difco™; Becton Dickinson and Company, Pont de Claix, France) and incubated at 22 ± 0·2°C or 37 ± 0·2°C for 48 and 24 h, respectively. Twenty presumptive Vibrio single colonies (yellow and green colonies) from each point were streaked onto TCBS agar and then isolated on Bacto™ heart infusion (HI) 2% NaCl agar medium, pH 7·4 (HI; Difco™; Becton Dickinson and Company) to control purity. They were enriched in 1·5 ml HI 2% NaCl liquid medium at 22 ± 0·2°C or 37 ± 0·2°C for 24 h, and the cultures were conserved on 96-well plates with 10% glycerol and at −80°C for further analyses.
The strains' DNA extracts were obtained according to Tall et al. (2012). Briefly, the strains stored at −80°C were newly cultured in a 2-ml 96-well plate containing 1·5 ml of HI 2% NaCl liquid medium per well and incubated 24 h at 22 ± 0·2°C or 37 ± 0·2°C, depending on the previous isolation temperature. Bacterial cultures were centrifuged, and pellets were washed twice with sterile buffered saline at 2000 g for 10 min and suspended in 100 μl of sterile distilled water and transferred to a 96-well microplate. After heating at 100°C for 15 min, the supernatants containing the DNA extracts were obtained by centrifugation at 3500 g for 10 min. The DNA extracts were diluted to obtain a final concentration of 50 ng μl−1 and stored at −20°C until use.
All the DNA extracts from presumptive Vibrio strains, showing phenotypic features of Vibrio on TCBS agar (yellow or green colonies), were tested by q-PCR SYBR Green (Invitrogen Kit; Fischer Scientific SAS, Illkirch Graffenstaden, France) using primers selected in the 16S rRNA region specific for the Vibrio genus (Thompson et al. 2004b). All the strains identified as belonging to the genus Vibrio spp. were then identified at the species level, by specific q-PCR concerning the 37°C strains or by partial sequencing in the cases where the q-PCR could not provide identification and for the 22°C strains. Vibrio cholerae, V. vulnificus and V. alginolyticus were identified using SYBR Green q-PCR systems targeting the dnaJ gene (Tall et al. 2012). Vibrio harveyi was identified using a SYBR Green q-PCR system targeting the toxR gene (A. Tall, L. Segon, A. Touron-Bodilis, and D. Hervio-Heath, unpublished data) designed with primers previously used by Pang et al. (2006).
The conditions of the following conventional PCR protocols were slightly modified from those described previously by Chimetto et al. (2009) and Pascual et al. (2010). A region of the pyrH gene (440 nt) was amplified by conventional PCR using the forward primer pyrH80F (5′GAT CGT ATG GCT CAA GAA G3′) and the reverse primer pyrH530R (5′TAG GCA TTT TGT GGT CAC G3′) as previously described by Chimetto et al. (2009). The amplification reactions contained 1× PCR buffer, 0·2 mmol l−1 of each deoxyribonucleotide triphosphate, 2 U Taq polymerase (Roche Diagnostics, Meylan, France), 0·48 μmol l−1 of each primer (Eurogentec, Seraing, Belgium) and 2 μl of the template (50 ng μl−1) or of sterile water (negative control) in a final reaction volume of 50 μl. The thermal programme consisted of a 5-min initial denaturation step at 95°C, followed by 30 cycles at 95°C for 1 min, 65°C for 2 min 15 s and 72°C for 1 min 15 s, and a final elongation step of 7 min at 72°C. A region of the toxR gene (477 nt) was amplified by conventional PCR using the forward primer toxRs (5′-GANCARGGNTTYGARGTNGAYGAYTC-3′) and the reverse primer toxRas (5′-TTDKKTTGNCCNCYNGTVGCDATNAC-3′) as previously described by Pascual et al. (2010). The thermal programme consisted of a 5-min initial denaturation step at 95°C, followed by 30 cycles at 95°C for 1 min, 61°C for 2 min 15 s and 72°C for 1 min 15 s, and a final elongation step of 7 min at 72°C. The quality of the amplified products was examined after an electrophoresis on 1·5% (w/v) agarose gel (Eurobio, Courtaboeuf, France) with 500 μg ml−1 ethidium bromide (Sigma-Aldrich®, Saint-Quentin Fallavier, France) staining in 1× Tris–borate–EDTA (TBE) electrophoresis buffer (Sigma-Aldrich®). The PCR products were then sent to the genomic platform of Biogenouest® (Station Biologique de Roscoff, Roscoff, France, www.sb-roscoff.fr) for purification and double-strand sequencing. The fragments purified with the Illustra™ ExoStar 1-Step (Dominique Dutscher SAS, Brumath, France) were used directly for DNA sequencing with the dideoxy chain termination method (BigDye® terminator cycle sequencing kit v3.1; Applied Biosystems, Life Technologies, Carlsbad, CA, USA) using the above-mentioned pyrH or toxR primers. The reaction products were analysed with the abi sequence scanner version 1.0 (Applied Biosystems, Life Technologies).
Sequence nucleotide alignments were first revised visually to identify positions with uncertain results, mainly at the 3′ and 5′ ends of the sequences, to be corrected or omitted for further analyses. Multiple sequence alignments were obtained using ClustalX (Thompson et al. 1997) with the bioedit v220.127.116.11. software (Hall 1999), taking into account the corresponding amino acid alignment for protein-coding genes with the seaview 4 software (Gouy et al. 2010). The phylogenetic constructions of both genes were inferred using the neighbour-joining (NJ) method (Saitou and Nei 1987) using Kimura-2-parameters distance estimations (Kimura 1980) and conducted with the mega 5 software (Tamura et al. 2011). The maximum intraspecies and interspecies distances were determined through distance matrix computed with the Kimura 2-parameter model, which involved 105 sequences for pyrH and 71 sequences for toxR. No gap or missing data were observed for any of the pyrH sequences. All positions containing alignment gaps and missing data were eliminated by pairwise deletions for toxR gene. Clades were drawn based on a bootstrap analysis with the figtree v1.3.1 program. The sequences from the strains isolated in this study were aligned with a wide range of pyrH and toxR sequences from reference or type strains of Vibrio species available on GenBank/EMBL, TAXVIBRIO (Thompson et al. 2009b) and StrainInfo websites (Dawyndt et al. 2005). These sequence data have been submitted to the GenBank database under accession No. JX401575–JX401899 and No. JX401900–JX401924 for pyrH and toxR, respectively.
Rarefaction curves were calculated to evaluate the representativeness of the number of colonies isolated per point, to picture the culturable Vibrio diversity at 22 and 37°C. A good representativeness is characterized when a plateau is reached, meaning that no increase in the number of species was detected when isolating more colonies. These analyses were performed using the past v1.68 software (Hammer et al. 2001).
Three hundred and fifteen strains were isolated at 22°C and they were all confirmed as belonging to the Vibrio genus by q-PCR identification. Among the strains isolated at 22°C, 291 (92·3%) were well identified through the partial sequencing of pyrH and could be assigned to twenty Vibrio species (Fig. 2). Vibrio celticus was the most frequently detected Vibrio species, with V. atlanticus and V. chagasii (36·8, 14 and 10·7% of the strains, respectively) (Fig. 3b). Vibrio crassostreae, V. kanaloae, V. cyclitrophicus, V. splendidus, V. rotiferianus, V. harveyi and V. fischeri species represented 2·2–6% of the strains, with more than ten strains per cluster (Fig. 3b). Among the 24 nonidentified strains after pyrH partial sequencing, one strain (W2 3G12 – GenBank accession number JX401717 and JX401908 for pyrH and toxR, respectively) showed 94% pyrH sequence similarity with Vibrio L2 sp. nov. strain R-77 (Chimetto et al. 2009). The toxR partial sequencing for this strain showed 98% sequence similarity with V. cyclitrophicus LMG 21580. The partial sequencing of pyrH and toxR did not allow the identification at the species level of the remaining 23 strains (7·3% of the total strains). These 23 strains were distributed among nine groups showing different percentages of pyrH sequence similarity (85–95%) with known Vibrio species (Table 1). The majority of those strains were related to the Splendidus group (seven strains) and to the Photobacterium group (12 strains).
|Group||Strains||Bootstrap||pyrH sequence similarities (%)|
|1||W 2 3E6||99||92–93% V. splendidus related species|
|S 1 5B9|
|W 1 6D2|
|S 2 6E2|
|2||W 1 4F4||93–94% V. chagasii|
|3||W 1 1A5||93–95% V. splendidus related species|
|4||W 1 4H6||91% V. orientalis related species and V. atypicus|
|5||W 2 1H2||92–93% V. splendidus related species|
|6||W 2 1G8||87–88% V. orientalis/V. splendidus related species and V. tapetis|
|7||W 2 1C1||97||85–86% V. anguillarum and V. campbelli|
|S 2 3A3|
|8||W 2 1F1||85–86% Photobacterium related species|
|9||W 2 1C8||98||85–87% Photobacterium related species|
|W 2 1E9|
|S 2 3A1|
|S 2 3D2|
|W 2 3G8|
|S 2 3E4|
|W 1 4E4|
|W 1 4F1|
|W 1 4B8|
|W 1 4G6|
|S 1 5C5|
After the q-PCR assays, only ten of the 37°C strains (n = 356) remained not identified. The partial sequencing of pyrH gene led to the assignment of two strains to the V. parahaemolyticus species (97–100% sequence similarities with V. parahaemolyticus pyrH sequences) (see in supplementary materials Fig. S1). Eight strains remained nonidentified by the partial sequencing of pyrH. The phylogenetic construction on the basis of the toxR gene led to assign seven strains to the Vibrio campbelli species and a single strain (10G9) to the Vibrio communis species (see in supplementary materials Fig. S2). Thus 23 species were detected among the 22 and 37°C strains (20 species at 22°C, six species at 37°C and three species in common). The analysis of the spatial distribution of the strains, performed on the phylogenetic trees obtained at 22°C and according to the origin of isolation (seawater or surface sediments, Transect 1 or Transect 2), revealed that the species were not specifically allocated to any of the compartments (seawater or sediment, data not shown). The diversity observed at 22 and 37°C revealed that if V. alginolyticus was predominant among the strains isolated at 37°C (85·9%), it became a minor species at 22°C and represented only 1·3% of the strains (Fig. 3a,b). Moreover, the number of Vibrio species isolated at 22°C (20 species) was higher than at 37°C (six species). Rarefaction curves for the different sampling points according to the number of species detected were calculated for the two transects and for the two temperatures of isolation (Fig. 4). The estimates of species numbers indicated that the sampling effort performed in this study (20 colonies per point) contributed to a good picture of the culturable Vibrio diversity at 37°C but to an underestimation of the culturable Vibrio diversity at 22°C.
The objective to determine the culturable Vibrio diversity at 37°C (potential human pathogens), but also at 22°C (potential animal pathogens), was reached by combining q-PCR assays and the partial sequencing of pyrH and toxR genes. Moreover, rarefaction analyses showed that the diversity of culturable Vibrio at 37°C was well represented. However, the culturable diversity at 22°C was underestimated at this temperature, according to the rarefaction curves analyses, and suggesting that more bacterial colonies should be isolated (above 40 colonies per point at least) to better represent the diversity at this temperature. Consequently, q-PCR assays were not adapted for the identification of the 22°C strains, the dominant species at 37°C (V. alginolyticus and V. harveyi) and the potential human pathogens being under- or not represented at 22°C. The sequencing of pyrH and toxR was a more suitable approach for the determination of the 22°C culturable Vibrio diversity.
The pyrH threshold of at least 94% sequence similarity for strains of the same species (Thompson et al. 2005) could lead, in some cases, to misidentifications in Vibrio groups as it was previously noticed by Pascual et al. (2010). These authors showed that the taxonomic resolution of pyrH was lower for some of the species of the Harveyi group, such as V. campbelli and V. rotiferianus, because they formed a polyphyletic group. In our study, the pyrH range of intraspecific and interspecific sequence similarities for the Harveyi group was 91·9–100% and 81·3–100%, respectively. This species delineation for the pyrH gene was determined through the analysis of 105 Vibrio sequences, from reference and environmental strains, of the Harveyi group. It revealed a low discriminatory level between the species V. campbelli and V. rotiferianus but also with the species V. harveyi, V. communis and V. owensii (up to 100% interspecific sequence similarities). Recently, Hoffmann et al. (2011) pointed Vibrio species misidentification within the Harveyi clade, particularly for V. communis and V. owensii, which would belong to the same species. The second gene selected for partial sequencing, the toxR gene, led to overcome the limit of pyrH observed within the Harveyi group. Indeed, the partial sequencing of the toxR gene for the eight remaining nonidentified strains isolated at 37°C showed a better taxonomic resolution, as confirmed by the gap observed between the maximum interspecies (31–70·2%) and the minimum intraspecies distances (77·4–100%). In this phylogenetic analysis, the position of the V. communis strain (LMG 25430 = R40496) within the V. campbelli group would suggest that V. communis and V. campbelli belong to the same species and thus this raises doubts about the identification of the 10G9 strain as V. communis. Even if previously described as the best phylogenetic marker, the toxR gene did not allow the identification of 23 of the 24 nonidentified strains within the 22°C pyrH phylogenetic tree. This could probably be explained by the lack of available Vibrio species sequences in the genomic banks for the toxR gene, which is still used seldomly as an identification tool for Vibrio. Another hypothesis is the presence of novel species among the nonidentified strains because sequence similarities were recorded below the minimum intraspecies similarities of the known species. Other targets could be selected to confirm this hypothesis, for example the rpoD gene that proved to be a better phylogenetic marker than pyrH (Pascual et al. 2010) and that was already used to describe novel species (Le Roux et al. 2005; Dieguez et al. 2011). Indeed, the relatedness of many Vibrio species led authors to find numerous phylogenetic markers to better discriminate closely related species (Thompson et al. 2007, 2008).
During this past decade, the MLSA raised the question of the notion of species or groups of species within the Vibrio genus (Sawabe et al. 2007; Thompson et al. 2007, 2009a; Pascual et al. 2010). Thus, Sawabe et al. (2007) described 14 groups or clades with MLSA and other phylogenetic and genetic markers (DNA–DNA hybridization, GC%, Amino Acid Identity, Phi test, radiation time and Habitat): the groups Anguillarum, Cholerae, Coralliilyticus, Diazotrophicus, Gazogenes, Fischeri, Halioticoli, Harveyi, Nereis, Nigripulchritudo, Orientalis, Scopthalmi, Splendidus and Vulnificus. Considering the taxonomic clustering applied to the 22 species identified in this study, the Splendidus group, including Vibrio celticus, V. crassostreae, V. chagasii, V. cyclitrophicus, V. gallaecicus, V. gigantis, V. kanaloae, V. lentus, V. pomeroyi, V. splendidus and V. tasmaniensis species, constituted the main group of the culturable Vibrio detected at 22°C (80% of the strains). It is also the dominant group of Vibrio in the coastal marine seawater, sediments (Sobecky et al. 1998; Urakawa et al. 1999, 2000; Radjasa et al. 2001) and bivalve molluscs (Le Roux and Austin 2006) worldwide. It comprises the highest number of species (above 10) with some pathogenic members for fish, bivalve molluscs or crustaceans (Beaz-Hidalgo et al. 2010). Vibrio celticus, the main species isolated in this study at 22°C (36·8%), and V. atlanticus (14%) were previously described as potentially pathogenic for clams (Beaz-Hidalgo et al. 2010; Dieguez et al. 2011). The third main species detected, V. chagasii (10·7%), has been isolated from fish larvae, rotifer and seawater (Thompson et al. 2003). The Harveyi group, including Vibrio alginolyticus, V. campbelli, V. harveyi, V. natriegens, V. parahaemolyticus and V. rotiferianus, represented the main group at 37°C (99·5% of the strains), the Cholera group being the second one.
Among the 37°C strains, the predominance of V. alginolyticus in the area is consistent with previous studies performed in Northern European waters (Hervio-Heath et al. 2002; Oberbeckmann et al. 2010). This species is considered as an opportunistic pathogen, rarely involved in human infections and not at the origin of severe cases. The clinical cases associated with V. alginolyticus are very rare and if any, the virulence factors triggering the illnesses are not clearly established for this species yet (Tantillo et al. 2004; Schets et al. 2006; Campanelli et al. 2008). Vibrio alginolyticus is part of the natural microflora of marine organisms but it could constitute a threat as this species has been reported in diseased corals, fishes, crustaceans and bivalves molluscs (Balebona et al. 1998; Kumar et al. 2007; Wang et al. 2007; Yan et al. 2007; Cervino et al. 2008; Harriague et al. 2008; Lafisca et al. 2008; Austin 2010). Moreover, it has been also associated with mortalities in aquaculture (Darbas et al. 1992; Snoussi et al. 2008). Unlike others studies, the second main species detected at 37°C is V. harveyi. This species is a luminescent bacterium associated with surface tissues, luminescent organs and digestive system of numerous marine organisms (Liu and Lee 1999). It was also associated with shrimp and fish mortalities (Alvarez et al. 1998; Liu and Lee 1999; Zhang and Austin 2000; Austin and Zhang 2006).
The comparison of the relative proportion of species between the two isolation temperatures showed that the potentially human pathogenic Vibrio detected at 37°C represented a very small fraction of the culturable diversity observed at 22°C. As an illustration, V. alginolyticus, representing 86% of the strains isolated in September 2009 at 37°C, represented only 1·3% of the strains isolated at 22°C. In a perspective to better evaluate the environmental risk linked to the presence of human and animal pathogenic vibrios, the simultaneous isolation at two temperatures could improve the understanding of the dynamics of their diversity and their ecology. Moreover, the dominance of pathogenic species for marine organisms belonging mainly to the Splendidus group raised the interest to explore the presence and the expression of virulence markers among these species. The determination of the environmental features associated with the analysis of the virulence genes distribution of these species could improve the risk assessment for aquaculture industries (Cano-Gomez et al. 2009).
In this study, pyrH and toxR genes proved to be appropriate targets for the screening of the culturable Vibrio diversity in the environment, particularly for the strains isolated at 22°C. In addition, the diversity analysis allowed picturing part of the potentially human pathogens among the total Vibrio population isolated at both temperatures. Moreover, the isolation of strains remains necessary to study pathogenicity mechanisms and simply the opportunity to identify new species. In a context of a routine monitoring and in the perspective of the evaluation of the potential risk of Vibrio for the marine organisms and for humans, the isolation at two temperatures could constitute a relevant transition step before the development of reliable direct detection and quantification methods of pathogenic Vibrio species in environmental samples.
This work was funded by the Vibrio Manche program of EDF R&D, the French Institute of research on marine exploration (Ifremer) and Eurofins IPL Nord. We would like to thank Dr Jean Louis Nicolas (Ifremer), Dr Marie-Anne Cambon-Bonavita (Ifremer) and Valérie Cueff-Gauchard (Ifremer) for providing Vibrio strains useful for this study. We also would like to thank Edith Delesmont (Eurofins IPL Nord), Olivier Mabille (EDF R&D), Lamine Barbach (EDF R&D), Joffrey Cholet, Margot Legoff, David Richard (Ifremer) and Gérard Morel (EDF R&D) for technical assistance. Finally, we would like to thank Alexandre Cebeillac (Ifremer) for his assistance for the Fig. 1 (map of the study area). We are most grateful to the Biogenouest® Genomics core facility and to the ABiMS® bioinformatic core facility for their technical support.