Development of amplified fragment length polymorphism (AFLP)-derived specific primer for the detection of Fusarium solani aetiological agent of peanut brown root rot

Authors

  • F. Casasnovas,

    1. Fellowship CONICET, Buenos Aires, Argentina
    2. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico- Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
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  • E.N. Fantini,

    1. Fellowship CONICET, Buenos Aires, Argentina
    2. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico- Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
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  • J.M. Palazzini,

    1. Fellowship CONICET, Buenos Aires, Argentina
    2. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico- Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
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  • G. Giaj-Merlera,

    1. Fellowship CONICET, Buenos Aires, Argentina
    2. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico- Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
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  • S.N. Chulze,

    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico- Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
    2. Members of the Research Career of CONICET, Buenos Aires, Argentina
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  • M.M. Reynoso,

    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico- Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
    2. Members of the Research Career of CONICET, Buenos Aires, Argentina
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  • A.M. Torres

    Corresponding author
    1. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico- Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina
    2. Members of the Research Career of CONICET, Buenos Aires, Argentina
    • Fellowship CONICET, Buenos Aires, Argentina
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Correspondence

Adriana M. Torres, Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional Nº 36, Km 601 (5800) Río Cuarto, Córdoba, Argentina. E-mail: atorres@exa.unrc.edu.ar

Abstract

Aims

The objective of this work was to design an amplified fragment length polymorphism (AFLP)-derived specific primer for the detection of Fusarium solani aetiological agent of peanut brown root rot (PBRR) in plant material and soil.

Methods and Results

Specific primers for the detection of the pathogen were designed based on an amplified region using AFLPs. The banding patterns by AFLPs showed that isolates from diseased roots were clearly distinguishable from others members of the F. solani species complex. Many bands were specific to F. solani PBRR, one of these fragments was selected and sequenced. Sequence obtained was used to develop specific PCR primers for the identification of pathogen in pure culture and in plant material and soil. Primer pair FS1/FS2 amplified a single DNA product of 175 bp. Other fungal isolates occurring in soil, included F. solani non-PBRR, were not detected by these specific primers. The assay was effective for the detection of pathogen from diseased root and infected soils.

Conclusions

The designed primers for F. solani causing PBRR can be used in a PCR diagnostic protocol to rapidly and reliably detect and identify this pathogen.

Significance and Impact of the Study

These diagnostic PCR primers will aid the detection of F. solani causing PBRR in diseased root and natural infected soils. The method developed could be a helpful tool for epidemiological studies and to avoid the spread of this serious disease in new areas.

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