Proteome variability among Helicobacter pylori isolates clustered according to genomic methylation

Authors

  • I. Vitoriano,

    1. Faculdade de Engenharia, Universidade Católica Portuguesa, Rio de Mouro, Portugal
    Search for more papers by this author
  • J.M.B. Vítor,

    1. Faculdade de Farmácia, iMed.UL, Universidade de Lisboa, Lisbon, Portugal
    Search for more papers by this author
  • M. Oleastro,

    1. Departamento de Doenças Infeciosase Departamento de Genética Humana, Instituto Nacional Saúde Dr. Ricardo Jorge, Rio de Mouro, Portugal
    Search for more papers by this author
  • M. Roxo-Rosa,

    Corresponding author
    1. Departamento de Doenças Infeciosase Departamento de Genética Humana, Instituto Nacional Saúde Dr. Ricardo Jorge, Rio de Mouro, Portugal
    2. BioFIG-Center for Biodiversity, Functional & Integrative Genomics, Faculdade de Ciências, Universidade de Lisboa, Lisbon, Portugal
    • Faculdade de Engenharia, Universidade Católica Portuguesa, Rio de Mouro, Portugal
    Search for more papers by this author
  • F.F. Vale

    Corresponding author
    • Faculdade de Engenharia, Universidade Católica Portuguesa, Rio de Mouro, Portugal
    Search for more papers by this author

Correspondence

Mónica Roxo-Rosa, Instituto Nacional Saúde Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisboa, Portugal. E-mail: roxorosa@hotmail.com

and

Filipa F. Vale, Faculdade de Engenharia, Universidade Católica Portuguesa, Estrada Octávio Pato, 2635-631 Rio de Mouro, Portugal. E-mail: filipavale@fe.lisboa.ucp.pt

Abstract

Aims

To understand whether the variability found in the proteome of Helicobacter pylori relates to the genomic methylation, virulence and associated gastric disease.

Methods and Results

We applied the Minimum-Common-Restriction-Modification (MCRM) algorithm to genomic methylation data of 30 Portuguese H. pylori strains, obtained by genome sensitivity to Type II restriction enzymes' digestion. All the generated dendrograms presented three clusters with no association with gastric disease. Comparative analysis of two-dimensional gel electrophoresis (2DE) maps obtained for total protein extracts of 10 of these strains, representative of the three main clusters, revealed that among 70 matched protein spots (in a universe of 300), 16 were differently abundant (< 0·05) among clusters. Of these, 13 proteins appear to be related to the cagA genotype or gastric disease. The abundance of three protein species, DnaK, GlnA and HylB, appeared to be dictated by the methylation status of their gene promoter.

Conclusions

Variations in the proteome profile of strains with common geographic origin appear to be related to differences in cagA genotype or gastric disease, rather than to clusters organized according to strain genomic methylation.

Significance and Impact of the Study

The simultaneous study of the genomic methylation and proteome is important to correlate epigenetic modifications with gene expression and pathogen virulence.

Ancillary