Proteome variability among Helicobacter pylori isolates clustered according to genomic methylation
Article first published online: 4 APR 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 6, pages 1817–1832, June 2013
How to Cite
Vitoriano, I., Vítor, J.M.B., Oleastro, M., Roxo-Rosa, M. and Vale, F.F. (2013), Proteome variability among Helicobacter pylori isolates clustered according to genomic methylation. Journal of Applied Microbiology, 114: 1817–1832. doi: 10.1111/jam.12187
- Issue published online: 17 MAY 2013
- Article first published online: 4 APR 2013
- Accepted manuscript online: 11 MAR 2013 03:15AM EST
- Manuscript Revised: 4 MAR 2013
- Manuscript Accepted: 4 MAR 2013
- Manuscript Received: 17 JAN 2013
- microbial phylogenetics;
- molecular genetic;
To understand whether the variability found in the proteome of Helicobacter pylori relates to the genomic methylation, virulence and associated gastric disease.
Methods and Results
We applied the Minimum-Common-Restriction-Modification (MCRM) algorithm to genomic methylation data of 30 Portuguese H. pylori strains, obtained by genome sensitivity to Type II restriction enzymes' digestion. All the generated dendrograms presented three clusters with no association with gastric disease. Comparative analysis of two-dimensional gel electrophoresis (2DE) maps obtained for total protein extracts of 10 of these strains, representative of the three main clusters, revealed that among 70 matched protein spots (in a universe of 300), 16 were differently abundant (P < 0·05) among clusters. Of these, 13 proteins appear to be related to the cagA genotype or gastric disease. The abundance of three protein species, DnaK, GlnA and HylB, appeared to be dictated by the methylation status of their gene promoter.
Variations in the proteome profile of strains with common geographic origin appear to be related to differences in cagA genotype or gastric disease, rather than to clusters organized according to strain genomic methylation.
Significance and Impact of the Study
The simultaneous study of the genomic methylation and proteome is important to correlate epigenetic modifications with gene expression and pathogen virulence.