Biofilm inhibitory and eradicating activity of wound care products against Staphylococcus aureus and Staphylococcus epidermidis biofilms in an in vitro chronic wound model
Article first published online: 4 APR 2013
© 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 114, Issue 6, pages 1833–1842, June 2013
How to Cite
Brackman, G., De Meyer, L., Nelis, H.J. and Coenye, T. (2013), Biofilm inhibitory and eradicating activity of wound care products against Staphylococcus aureus and Staphylococcus epidermidis biofilms in an in vitro chronic wound model. Journal of Applied Microbiology, 114: 1833–1842. doi: 10.1111/jam.12191
- Issue published online: 17 MAY 2013
- Article first published online: 4 APR 2013
- Accepted manuscript online: 12 MAR 2013 03:41PM EST
- Manuscript Revised: 7 MAR 2013
- Manuscript Accepted: 7 MAR 2013
- Manuscript Received: 4 JAN 2013
- Interuniversity Attraction Poles Programme. Grant Numbers: Ghent University, BOF09/GOA/011
- Ghent University. Grant Number: BOF09/GOA/011
- biofilm eradication;
- biofilm inhibition;
- chronic wounds;
- Staphylococcus spp
Although several factors contribute to wound healing, bacterial infections and the presence of biofilm can significantly affect healing. Despite that this clearly indicates that therapies should address biofilm in wounds, only few wound care products have been evaluated for their antibiofilm effect. For this reason, we developed a rapid quantification approach to investigate the efficacy of wound care products on wounds infected with Staphylococcus spp.
Methods and Results
An in vitro chronic wound infection model was used in which a fluorescent Staph. aureus strain was used to allow the rapid quantification of the bacterial burden after treatment. A good correlation was observed between the fluorescence signal and the bacterial counts. When evaluated in this model, several commonly used wound dressings and wound care products inhibited biofilm formation resulting in a decrease between one and seven log CFU per biofilm compared with biofilm formed in the absence of products. In contrast, most dressings only moderately affected mature biofilms.
Our model allowed the rapid quantification of the bacterial burden after treatment. However, the efficacy of treatment varied between the different types of dressings and/or wound care products.
Significance and Impact of the Study
Our model can be used to compare the efficacy of wound care products to inhibit biofilm formation and/or eradicate mature biofilms. In addition, the results indicate that treatment of infected wounds should be started as soon as possible and that novel products with more potent antibiofilm activity are needed.