Molecular characterization of Streptococcus agalactiae strains isolated from fishes in Malaysia
Article first published online: 18 APR 2013
Journal of Applied Microbiology © 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 1, pages 20–29, July 2013
How to Cite
Amal, M.N.A., Zamri-Saad, M., Siti-Zahrah, A., Zulkafli, A.R. and Nur-Nazifah, M. (2013), Molecular characterization of Streptococcus agalactiae strains isolated from fishes in Malaysia. Journal of Applied Microbiology, 115: 20–29. doi: 10.1111/jam.12210
- Issue published online: 12 JUN 2013
- Article first published online: 18 APR 2013
- Accepted manuscript online: 5 APR 2013 03:43AM EST
- Manuscript Accepted: 27 MAR 2013
- Manuscript Revised: 22 MAR 2013
- Manuscript Received: 8 FEB 2013
- RAPD ;
- Streptococcus agalactiae
The aim of this study was to characterize Streptococcus agalactiae strains that were isolated from fishes in Malaysia using random amplified polymorphic DNA (RAPD) and repetitive extragenic palindromic PCR (REP-PCR) techniques.
Methods and Results
A total of 181 strains of Strep. agalactiae isolated from red hybrid tilapia (Oreochromis sp.) and golden pompano (Trachinotus blochii) were characterized using RAPD and REP-PCR techniques. Both the fingerprinting techniques generated reproducible band patterns, differing in the number and molecular mass amplicons. The RAPD technique displayed greater discriminatory power by its production of more complex binding pattern and divided all the strains into 13 groups, compared to 9 by REP-PCR technique. Both techniques showed the availability to differentiate the genetic profiles of the strains according to their geographical location of origin. Three strains of Strep. agalactiae that were recovered from golden pompano showed a genetic dissimilarity from the strains isolated from red hybrid tilapia, while the strain of ATCC 27956 that recovered from bovine displayed a unique profile for both methods.
Both techniques possess excellent discriminative capabilities and can be used as a rapid means of comparing Strep. agalactiae strains for future epidemiological investigation.
Significance and Impact of the Study
Framework as the guideline in traceability of this disease and in the search for potential local vaccine candidates for streptococcosis in this country.