Herd prevalence of Enterobacteriaceae producing CTX-M-type and CMY-2 β-lactamases among Japanese dairy farms
Article first published online: 17 APR 2013
Journal of Applied Microbiology © 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 1, pages 282–289, July 2013
How to Cite
Ohnishi, M., Okatani, A.T., Esaki, H., Harada, K., Sawada, T., Murakami, M., Marumo, K., Kato, Y., Sato, R., Shimura, K., Hatanaka, N. and Takahashi, T. (2013), Herd prevalence of Enterobacteriaceae producing CTX-M-type and CMY-2 β-lactamases among Japanese dairy farms. Journal of Applied Microbiology, 115: 282–289. doi: 10.1111/jam.12211
- Issue published online: 12 JUN 2013
- Article first published online: 17 APR 2013
- Accepted manuscript online: 1 APR 2013 09:38AM EST
- Manuscript Accepted: 27 MAR 2013
- Manuscript Revised: 11 MAR 2013
- Manuscript Received: 6 JAN 2013
- CMY-2 β-lactamase;
- CTX-M type ESBLs;
- dairy cattle;
- Escherichia coli ;
- multilocus sequence typing;
- pulsed-field gel electrophoresis
To determine the herd prevalence of Enterobacteriaceae producing CTX-M-type extended-spectrum β-lactamases (ESBLs) among 381 dairy farms in Japan.
Methods and Results
Between 2007 and 2009, we screened 897 faecal samples using BTB lactose agar plates containing cefotaxime (2 μg ml−1). Positive isolates were tested using ESBL confirmatory tests, PCR and sequencing for CTX-M, AmpC, TEM and SHV. The incidence of Enterobacteriaceae producing CTX-M-15 (n = 7), CTX-M-2 (n = 12), CTX-M-14 (n = 3), CMY-2 (n = 2) or CTX-M-15/2/14 and CMY-2 (n = 4) in bovine faeces was 28/897 (3·1%) faecal samples. These genes had spread to Escherichia coli (n = 23) and three genera of Enterobacteriaceae (n = 5). Herd prevalence was found to be 20/381 (5·2%) dairy farms. The 23 E. coli isolates showed clonal diversity, as assessed by multilocus sequence typing and pulsed-field gel electrophoresis. The pandemic E. coli strain ST131 producing CTX-M-15 or CTX-M-27 was not detected.
Three clusters of CTX-M (CTX-M-15, CTX-M-2, CTX-M-14) had spread among Japanese dairy farms.
Significance and Impact of the Study
This is the first report on the prevalence of multidrug-resistant CTX-M-15–producing E. coli among Japanese dairy farms.