Bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of Bikalga, an alkaline fermented food
Article first published online: 30 APR 2013
Journal of Applied Microbiology © 2013 The Society for Applied Microbiology
Journal of Applied Microbiology
Volume 115, Issue 1, pages 133–146, July 2013
How to Cite
Compaoré, C.S., Nielsen, D.S., Sawadogo-Lingani, H., Berner, T.S., Nielsen, K.F., Adimpong, D.B., Diawara, B., Ouédraogo, G.A., Jakobsen, M. and Thorsen, L. (2013), Bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of Bikalga, an alkaline fermented food. Journal of Applied Microbiology, 115: 133–146. doi: 10.1111/jam.12214
- Issue published online: 12 JUN 2013
- Article first published online: 30 APR 2013
- Accepted manuscript online: 9 APR 2013 06:34AM EST
- Manuscript Accepted: 2 APR 2013
- Manuscript Revised: 4 MAR 2013
- Manuscript Received: 15 DEC 2012
- Danish International Development Agency
- antimicrobial activity;
- Bacillus amyloliquefaciens ssp. plantarum;
- starter cultures
To identify and screen dominant Bacillus spp. strains isolated from Bikalga, fermented seeds of Hibiscus sabdariffa for their antimicrobial activities in brain heart infusion (BHI) medium and in a H. sabdariffa seed-based medium. Further, to characterize the antimicrobial substances produced.
Methods and Results
The strains were identified by gyrB gene sequencing and phenotypic tests as B. amyloliquefaciens ssp. plantarum. Their antimicrobial activity was determined by the agar spot and well assay, being inhibitory to a wide range of Gram-positive and Gram-negative pathogenic bacteria and fungi. Antimicrobial activity against Bacillus cereus was produced in H. sabdariffa seed-based medium. PCR results revealed that the isolates have potential for the lipopeptides iturin, fengycin, surfactin, the polyketides difficidin, macrolactin, bacillaene and the dipeptide bacilysin production. Ultra-high-performance liquid chromatography-time of flight mass spectrometry analysis of antimicrobial substance produced in BHI broth allowed identification of iturin, fengycin and surfactin.
The Bacillus amyloliquefaciens ssp. plantarum exhibited broad-spectrum antifungal and antibacterial properties. They produced several lipopeptide antibiotics and showed good potential for biological control of Bikalga.
Significance and Impact of the Study
Pathogenic bacteria often occur in spontaneous food fermentations. This is the first report to identify indigenous B. amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for safeguarding Bikalga.